脱细胞人羊膜支架材料对羊膜源性干细胞增殖作用的影响

Effect of human acellular amniotic membrane scaffold material on the proliferation of amniotic membrane-derived stem cells

目的研究脱细胞人羊膜支架材料的制备及其对羊膜源性干细胞增殖作用的影响。方法利用38~40周孕妇剖宫产术后的羊膜,洗净后用物理及酶消化的方法清除羊膜细胞成分,得到脱细胞人羊膜支架材料,并制备了不同浓度的包被培养皿。剥离剖宫产后的羊膜利用流式细胞分选技术从中分离干细胞,并在包被培养皿上探讨细胞增殖活性,与其他生物材料包被培养皿做比较。结果羊膜源性干细胞在脱细胞人羊膜支架材料的包被培养皿上增殖速度明显增强,且与包被浓度明显相关。与常用的支架材料相比其对干细胞增殖效果更强,差异显著。结论脱细胞人羊膜支架材料有望成为干细胞研究与再生医疗领域中的新型生物材料。

Objective Preparation of human acellular amniotic membrane scaffold and its effect on the proliferation of amniotic membrane-derived stem cells.Methods The amniotic membrane of pregnant women after caesarean section at 38-40 weeks was used.The amniotic membrane cell components were removed by physical and enzymatic digestion after washing,and the acellular amniotic membrane scaffold material was obtained,and coated culture dishes of different concentrations were prepared.The amniotic membrane after the caesarean section was stripped and the stem cells were separated by flow cytometry.The amniotic membrane-derived stem cells were cultured on petri dish coated with acellular amniotic membrane scaffold and the cell proliferation activity was analyzed by Cell Counting Kit-8(CCK-8),and compared with other biological materials coated petri dishes.Results The proliferation rate of stem cells on a culture dish coated with acellular amniotic membrane scaffold was significantly increased,which was significantly related to the coating concentration.Compared with commonly used scaffold materials,it has a stronger effect on stem cell proliferation,and the difference is significant.Conclusion Human acellular amniotic membrane scaffold material is expected to become a new type of biomaterial in the field of stem cell research and regenerative medicine.