不同移栽基质对苹果砧木B9无糖组培苗生长的影响

Effects of Different Transplanting Media on Growth of Sugar-Free Tissue Culture Seedlings of Apple Rootstock B9

以苹果砧木B9无糖组培苗为试材,研究了不同成分配比基质对无糖组培苗移栽的影响。结果表明:5种基质处理无糖组培苗B9的移栽成活率均达到100%。其中,以成品基质∶蛭石∶珍珠岩∶锯末=2∶1∶1∶1配比作为移栽基质,B9组培苗茎粗显著高于对照组,地上部长势较好,根系指标中根冠比、根系粗度、根系表面积、根系体积指标均最高,根长也显著高于对照组,根系最发达。综上,最适合根系生长的移栽基质配比为成品基质:蛭石:珍珠岩:锯末=2∶1∶1∶1。

With apple rootstock B9 sugar-free tissue culture seedlings as the test material,the effects of different composition ratio substrates on the transplanting of sugar-free tissue culture seedlings were studied.The results showed that the transplanting survival rate of sugar-free tissue culture seedlings B9 reached 100%under treatments of the five substrates.Among them,with the finished substrate∶vermiculite∶perlite∶sawdust=2∶1∶1∶1,the stem thickness of the B9 group cultured seedlings was significantly higher than that of the control group,the ground growth was better.The root/shoot ratio,root thickness,root surface area,and root volume were the highest among the root indexes.The root length was also significantly higher than the control group,and the root system was the most developed.In summary,the most suitable transplanting substrate ratio for root growth is the finished substrate∶vermiculite∶perlite∶sawdust=2∶1∶1∶1.