常间回文重复序列丛集/关联蛋白系统系统靶向长链非编码核糖核酸在肿瘤研究中的应用进展

Research progress in the application of clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeats-associated proteins system targeting long non-coding RNAs in tumor

长链非编码核糖核酸(lncRNAs)在恶性肿瘤发生和发展的调控中发挥重要作用,为多种肿瘤治疗研究中新的靶点。常间回文重复序列丛集(CRISPR)/CRISPR关联蛋白系统(Cas)系统为新近发展的高效精准的基因组编辑工具。多项研究表明CRISPR/Cas系统可靶向lncRNAs,为肿瘤细胞lncRNAs表达的调控提供了新策略。本文针对CRISPR/Cas技术调控lncRNA功能的机制及其在恶性肿瘤研究中的进展进行综述。

Long non-coding ribonucleic acids(lncRNAs)play an important role in the regulation of malignant tumor genesis and development,which is a new potential target for cancer treatment research.Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated proteins(Cas)system is a revolutionary genome editing tool developed in recent year with high efficiency and accuracy.A growing number of studies have shown that CRISPR/Cas system can target lncRNAs,which could provide a new strategy for regulating the expression of lncRNAs in tumor cells.We reviewed the mechanism of CRISPR/Cas technology regulating lncRNA function and its new progress in malignant tumor research.