摘要
Small interfering RNA(siRNA)constitutes a promising therapeutic modality supporting the potential functional cure of hepatitis B.A novel ionizable lipidoid nanoparticle(RBP131)and a state-of-the-art lyophilization technology were developed in this study,enabling to deliver siRNA targeting apolipoprotein B(APOB)into the hepatocytes with an ED_(50)of 0.05 mg/kg after intravenous injection.In addition,according to the requirements of Investigational New Drug(IND)application,a potent siRNA targeting hepatitis B virus(HBV)was selected and encapsulated with RBP131 to fabricate a therapeutic formulation termed RB-HBV008.
基金
This work was supported by the National Drug Program of China(2012ZX09102301-006,2015ZX09102-023-002,2014ZX09304313-001,and 2009ZX09103732)
the National Natural Science Foundation of China(31871003)
the Beijing-Tianjin-Hebei Basic Research Cooperation Project(19JCZDJC64100)
the Beijing Nova Program from Beijing Municipal Science&Technology Commission(Z201100006820005).
