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基于UHPLC-Q-TOF-MS/MS技术探究和表征杜仲提取物在腺嘌呤致肾纤维化大鼠中的入血成分及其代谢产物 被引量:6

Profiling and characterization of the ingredients absorbed into blood and their metabolites of the Eucommiae Cortex extracts in rats with renal fibrosis induced by adenine based on UHPLC-Q-TOF-MS/MS
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摘要 探究和表征杜仲提取物在腺嘌呤致肾纤维化大鼠中的入血成分,为其药效物质基础研究提供参考。以腺嘌呤致肾纤维化SD大鼠为实验对象,灌胃给予杜仲提取物并收集血浆样品,利用超高效液相色谱-四极杆-飞行时间串联质谱(UHPLC-Q-TOF-MS/MS)技术分析鉴定吸收入血的原形成分及其代谢产物。本实验获得南京中医药大学实验动物伦理委员会批准(批准号:202103A008)。结果共鉴定出入血原形成分24种,其中木脂素类9种、环烯醚萜类4种、苯丙素类8种、有机酸类3种;进一步分析得到代谢产物30种,其中木脂素类9种、环烯醚萜类19种、有机酸类2种。研究结果可为进一步阐明杜仲治疗肾纤维化的药效物质基础及其作用机制提供有价值的依据。 To profile and characterize the ingredients absorbed into blood and their metabolites of the Eucommiae Cortex(EC) extracts in rats with renal fibrosis induced by adenine, and so as to provide a reference for investigation of the pharmacodynamic substances of EC. SD rats with renal fibrosis induced by adenine were intragastrically administered with the EC extracts, and the rat plasma samples were collected and analyzed by UHPLC-Q-TOF-MS/MS to identify the prototype ingredients absorbed into blood and their metabolites. The experiment was approved by the experimental Animal Ethics Committee from Nanjing University of Chinese Medicine(No. 202103A008). The results showed that a total of 24 prototype compounds were identified, including9 lignans, 4 iridoids, 8 phenylpropanoids, and 3 organic acids. Furthermore, 30 metabolites were obtained by further analysis, including 9 lignans, 19 iridoids, and 2 organic acids. The results of this study can provide the valuable reference for further elucidation of the pharmacodynamic substantial basis and mechanism of EC in the treatment of renal fibrosis.
作者 王梦晴 于慧 刘鑫 宋健涛 蔡皓 曹岗 朱慧 段煜 裴科 WANG Meng-qing;YU Hui;LIU Xin;SONG Jian-tao;CAI Hao;CAO Gang;ZHU Hui;DUAN Yu;PEI Ke(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing,Nanjing University of Chinese Medicine,Nanjing 210023,China;School of Pharmacy,Zhejiang Chinese Medical University,Hangzhou 310053,China;School of Chinese Medicine and Food Engineering,Shanxi University of Chinese Medicine,Jinzhong 030619,China)
出处 《药学学报》 CAS CSCD 北大核心 2022年第4期1136-1146,共11页 Acta Pharmaceutica Sinica
基金 国家自然科学基金优秀青年基金项目(81922073)。
关键词 杜仲 超高效液相色谱-四极杆-飞行时间串联质谱 入血成分 代谢产物 肾纤维化 Eucommiae Cortex UHPLC-Q-TOF-MS/MS ingredient absorbed into blood metabolite renal fibrosis
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