核黄素对砷诱导小鼠心、肺、脑毒性损伤的保护效应

Protective Effect of Riboflavin on Arsenic-induced Toxic Injury to the Heart,Lung and Brain in Mice

本试验通过饮水途径对48只5周龄小鼠进行砷暴露和核黄素干预,设置对照组、砷处理组(20 mg/L As_(2)O_(3))、核黄素处理组(20 mg/L VB_(2))及砷加核黄素联合组(20 mg/L As_(2)O_(3)+20 mg/L VB_(2)),探究核黄素对砷致器官组织损伤的缓解作用。结果显示,与对照组相比,砷处理会引起小鼠心脏心肌纤维间隔增宽,纤维束弯曲,心肌细胞核脱落,肺泡壁增厚,肺泡腔缩小,大脑皮层细胞数量明显减少,且排列紊乱,细胞核溶解破裂,而添加核黄素显著减轻了上述损伤。因此,本研究证实了核黄素干预对砷中毒引起的心、肺和脑组织结构损伤有明显的缓解作用,进一步为砷中毒的毒理机制和防治提供了新的思路。

In this experiment,485-week-old mice were exposed to arsenic and riboflavin through drinking water.The control group,the arsenic treatment group(20 mg/L As_(2)O_(3)),the riboflavin treatment group(20 mg/L VB_(2)),the arsenic and riboflavin combined group(20 mg/L As_(2)O_(3)+20 mg/L VB_(2))were set up to explore the alleviating effect of riboflavin on tissue injury caused by arsenic.The results showed that arsenic treatment caused the heart muscle fiber septa widening,fiber bundles bending,myocardial nucleus shedding,alveolar wall thickening and alveolar cavity shrinking compared with the control group.The number of cerebral cortex cells was significantly reduced and disordered,and the nuclei were dissolved and broken.The addition of riboflavin significantly reduced the damage of arsenic to heart,lung and brain.Therefore,this study confirmed that riboflavin intervention has a significant alleviating effect on the structural damage of heart,lung and brain caused by arsenic,it provides new ideas for the toxicological mechanism and prevention of arsenic poisoning.