肿瘤坏死因子α诱导蛋白3在胶质瘤中的表达特性及其对胶质瘤细胞侵袭和迁移的影响

Expression characteristics of tumor necrosis factor alpha inducible protein 3 in glioma and its effect on invasion and migration of glioma cell

目的探讨肿瘤坏死因子α诱导蛋白3(TNFAIP3)在胶质瘤中的表达及其对胶质瘤细胞侵袭、迁移的影响。方法采用实时荧光定量法(qRT-PCR)检测人星形胶质细胞NHA和胶质瘤细胞株U87、U251、SNB19、T98和LN229中TNFAIP3的表达,以U87和SNB19为实验对象。使用阳离子脂质体Lipofectamine 2000介导小干扰RNA(siRNA)转染U87和SNB19以下调TNFAIP3的表达;采用Transwell检测下调TNFAIP3对U87及SNB19侵袭的影响;运用划痕实验检测下调TNFAIP3对U87及SNB19迁移的影响。结果和NHA(0.144±0.008)相比,TNFAIP3在U87(1.380±0.066)、U251(0.663±0.062)、SNB19(1.405±0.032)、T98(1.002±0.068)、LN229(0.667±0.027)中的表达均升高(均P<0.05)。si-TNFAIP3转染U87及SNB19可下调TNFAIP3的表达,继续培养24 h,U87及SNB19侵袭和迁移能力均下降(均P<0.05)。结论TNFAIP3在胶质瘤中高表达,TNFAIP3可促进U87及SNB19的侵袭和迁移,si-TNFAIP3可抑制胶质瘤细胞的侵袭和迁移。

Objective To investigate the expression of tumor necrosis factor alpha inducible protein 3(TNFAIP3)in gliomas and its effect on the invasion and migration of glioma cells.Methods Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)was used to detect the expression of TNFAIP3 in normal human astrocytes NHA and glioma cell lines U87,U251,SNB19,T98 and LN229,respectively.U87 and SNB19 were used as experiments subjects.Lipofectamine 2000-mediated small interference RNA(siRNA)was used to transfect U87 and SNB19 to down-regulate the expression of TNFAIP3.The effect of down-regulated TNFAIP3 on the invasion of U87 and SNB19 was detected by Transwell,and the migration was detected by scratch test.Results Compared with NHA(0.144±0.008),the expression of TNFAIP3 in U87(1.380±0.066),U251(0.663±0.062),SNB19(1.405±0.032),T98(1.002±0.068)and LN229(0.667±0.027)were increased(all P<0.05).Si-TNFAIP3 transfection into U87 and SNB19 could down-regulate the expression of TNFAIP3 and continue to culture for 24 hours,the invasion and migration ability of U87 and SNB19 were decreased(all P<0.05).Conclusion TNFAIP3 is highly expressed in glioma,which can promote the invasion and migration of U87 and SNB19,and si-TNFAIP3 can inhibit the invasion and migration of glioma cells.