摘要
目的了解兽医相关单位实验室Ⅱ级生物安全柜的性能与使用情况。方法测试哈尔滨市3家兽医相关单位正在使用的126台Ⅱ级生物安全柜,依据相关标准针对检测结果进行性能指标的判定与分析,了解生物安全柜的使用情况。结果126台Ⅱ级生物安全柜性能检测总合格率为17.5%。Ⅱ级生物安全柜的外观完整性、工作区洁净度、送排风高效过滤器检漏、气流模式、流入气流流速、下降气流流速、照度、噪声及报警和联锁系统共9个项目的检测总合格率分别为100%、100%、100%、100%、49.2%、92.9%、68.3%、22.2%和100%。结论兽医相关单位实验室Ⅱ级生物安全柜性能检测总合格率较低,应对生物安全柜做定期性能检测,加强运行维护管理,及时排除生物安全隐患,防止实验室感染事故发生。
Objective To detect the performance and use of class Ⅱ biosafety cabinets in laboratories of veterinary-related research institutes.Methods A total of 126 class Ⅱ biological safety cabinetries being used by three veterinary-related reseatch institutes in Harbin were tested.The performance indicators were judged according to the test results and and analyze according to relevant standards,in order to understand the use of biological safety cabinets.Results The overall pass rate of 126 biosafety cabinetries was 17.5%.The total qualified rate were 100%,100%,100%,100%,49.2%,92.9%,68.3%,22.2%and 100%,respectively in terms of the appearance of integrity,cleanliness of work area,integrity of high efficiency filter for supply and exhaust air,flow pattern,the velocity of inflow,the velocity of the downdraft,noise,illumination and alarm and interlock system of class Ⅱ biosafety cabinet.Conclusion The overall pass rate of biosafety cabinetries in some veterinary-related research institutes is low.Regular inspection,operation and maintenance management should be strengthened to eliminate biological safety hazard timely and prevent infection accidents of laboratory.
作者
马文杰
张伟
王伟
苏世博
郭慧娟
张贺
王梓
刘怀然
陈洪岩
MA Wenjie;ZHANG Wei;WANG Wei;SU Shibo;GUO Huijuan;ZHANG He;WANG Zi;LIU Huairan;CHEN Hongyan(State Key Laboratory of Veterinary Biotechnology,National Poultry Laboratory Animal Resource Center,Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150069,China)
出处
《实验动物与比较医学》
CAS
2022年第2期111-116,共6页
Laboratory Animal and Comparative Medicine
基金
国家重点研发计划项目:BSL-4实验室IVC笼盒更换工作台的研制及应用(2018YFC1200301)
手套箱式生物隔离器和感染动物隔离设备的研发(2016YFC1201401)。
关键词
生物安全柜
性能检测
兽医实验室
Biosafety cabinetry
Parameters test
Veterinary laboratory