高效液相色谱法测定小活络丸中葡萄糖、果糖、蔗糖、麦芽糖

Determination of glucose,fructose,sucrose and maltose in Xiaohuoluo pills by high performance liquid chromatography

建立高效液相色谱法同时测定小活络丸中葡萄糖、果糖、蔗糖、麦芽糖含量的方法。以50%乙腈–水溶液作为提取溶剂,进样体积为20μL,以乙腈–水(体积比为75∶25)为流动相,流量为1.0 mL/min,采用ZORBAX Carbohydrate型色谱柱(250 mm×4.6 mm,5μm)进行分离,柱温为40℃;采用示差折光检测器检测,温度为40℃。果糖、葡萄糖、蔗糖、麦芽糖在各自的质量浓度范围内与色谱峰面积线性关系良好,相关系数为0.9992~0.9997,检出限与定量限分别为0.034~0.083、0.067~0.21 mg/mL。平均加标回收率为87.2%~106%,测定结果的相对标准偏差为1.5%~5.0%(n=6)。该方法操作简单,可用于小活络丸中葡萄糖、果糖、蔗糖、麦芽糖的测定,并对小活络丸的质量进行控制。

A high performance liquid chromatography(HPLC)method was established for the simultaneous determination of glucose,fructose,sucrose and maltose in Xiaohuoluo pills.Extracted with 50%acetonitrile-aqueoussolution,and the injection volume was 20μL.Acetonitrile water(volume ratio 75∶25)was used as the mobile phase,the flow rate was 1.0 mL/min,and Zorbax carbohydrate column(250 mm×4.6 mm,5μm)was used for separation,the column temperature was 40℃.Differential refraction detector was used for detection,and the temperature was 40℃.The correlation coefficient was 0.9992-0.9997,the detection limit and quantitative limit were 0.034-0.083 and 0.067-0.21 mg/mL,respectively.The average recovery was 87.2%-106%,and the relative standard deviation of the determination results was 1.5%-5.0%(n=6).The method is simple and can be used for the determination of glucose,fructose,sucrose and maltose in Xiaohuoluo pills,and the quality of Xiaohuoluo pills can be controlled.