山西小麦地方品种品质相关基因的KASP标记分析

Detection of Quality-Related Genes in the Wheat Landrace in Shanxi Province by KASP Markers

为发掘和利用小麦地方品种中的优良品质性状基因资源,本研究利用30个KASP分子标记对405份山西小麦地方品种的15个品质性状相关基因进行了检测。结果表明,在供试材料中籽粒硬度优异等位变异Pinb-D1b与Pinb-B2b分别占比为4.94%和63.46%;麦谷蛋白Glu-1位点的优异等位变异Glu-Ax1或Glu-Ax2*占比为93.09%,Glu-Bx7OE占比为47.16%,Glu-D1d占比为0.74%;面粉色泽相关的优异等位变异Psy-A1b占比为1.23%,Psy-B1a或Psy-B1b为95.80%,Psy-D1a为87.65%,TaPds-B1b为0.74%,Zds-A1a为87.16%,TaLcy-B1b为96.05%,Lox-B1a为88.89%,TaPod-A1b为1.73%,Ppo-A1b为97.28%,Ppo-D1a为20.94%。且在山西中部晚熟和南部中熟冬麦区中优异等位变异的频率分布范围分别为0.52%~97.41%、0~97.51%。此外,鉴定出89份材料聚合9个优异等位变异的组合Pinb-B2b/Glu-Ax1或Glu-Ax2*/Glu-Bx7OE/Psy-B1a或PsyB1b/Psy-D1a/Zds-A1a/TaLcy-B1b/Lox-B1a/Ppo-A1b,其频率为21.98%。本研究筛选出含有优异等位变异的小麦地方品种,为小麦品质相关性状的遗传改良提供了重要参考。

To explore and utilize the genetic resources of good quality traits in wheat landraces,15 quality trait-related genes in 405 wheat landraces of Shanxi province were genotyped using 30 KASP markers. The results showed that the average frequency of the favorable allelic variations Pinb-D1b and Pinb-B2b for grain hardness accounted for 4.94% and 63.46%,respectively. At the Glu-1 locus,the favorable alleles Glu-Ax1(or Glu-Ax2*),Glu-Bx7OE and Glu-D1d accounted for 93.09%,47.16% and 0.74%,respectively. Elite alleles for flour color including,Psy-A1b,Psy-B1a(or Psy-B1b),Psy-D1a,Ta Pds-B1b,Zds-A1a,Ta Lcy-B1b,Lox-B1a,Ta Pod-A1b,Ppo-A1b and Ppo-D1a accounted for 1.23%,95.80%,87.65%,0.74%,87.16%,96.05%,88.89%,1.73%,97.28% and 20.94%,respectively. The favorable allele frequncy of landraces,which were derived from the late-maturing winter wheat region in central Shanxi and the south mid-maturing winter wheat region,ranged from 0.52% to 97.41% and 0 to 97.51%,respectively. In addition,nine favorable alleles Pinb-B2b/Glu-Ax1(or Glu-Ax2*)/Glu-Bx7OE/Psy-B1a(or Psy-B1b)/Psy-D1a/Zds-A1a/TaLcy-B1b/Lox-B1a/Ppo-A1b were simultanously detected in 89 samples,with a frequency of 21.98%. By conducting marker-assisted identification of the excellent alleles in wheat landraces,this study provided reference for the genetic improvement at quality-related traits in bread wheat.