鼻咽癌组织的erb-b2受体酪氨酸激酶3水平及临床意义

Level and clinical significance of erb-b2 receptor tyrosine kinase 3 in nasopharyngeal carcinoma tissues

目的探讨鼻咽癌(NPC)组织的erb-b2受体酪氨酸激酶3(ErbB3)水平及临床意义。方法选取2013年1月至2015年9月65例经病理证实为NPC患者的癌组织及对应癌旁正常鼻咽部组织,采用实时荧光定量PCR(qPCR)和Western blotting检测NPC组织的ErbB3 mRNA和蛋白水平,分析ErbB3 mRNA水平与NPC临床病理特征和预后的关系。结果Western blotting结果显示,30例NPC组织的ErbB3蛋白水平高于对应癌旁组织(P<0.05);qPCR结果显示,65例NPC组织的ErbB3 mRNA水平为4.625±1.980,高于对应癌旁组织的1.033±0.600(P<0.05)。ErbB3 mRNA水平与NPC患者的性别、年龄、病理分型、肿瘤最大径和是否复发无关(P>0.05),而与肿瘤侵犯深度的T分期、淋巴结有无转移的N分期、有无远处转移的M分期和综合的TNM分期有关(P<0.05)。ErbB3 mRNA高水平组与低水平组的中位总生存期(OS)分别为52.0个月和未达,3年总生存率分别为75.0%和86.2%,5年总生存率分别为33.3%和65.5%,差异有统计学意义(P<0.05);多因素分析显示ErbB3 mRNA水平是影响NPC患者OS的独立风险因素(HR=2.907,95%CI:1.372~6.160,P=0.005)。结论ErbB3在NPC的发生发展中可能起至关重要作用,与预后密切相关,有望成为判断NPC预后的新指标及临床治疗的新靶点。

Objective To investigate the level and clinical significance of erb-b2 receptor tyrosine kinase 3(ErbB3)in nasopharyngeal carcinoma(NPC)tissues.Methods From January 2013 to September 2015,65 cases of NPC tissues confirmed by pathology and corresponding normal nasopharyngeal tissues adjacent to cancer were selected.The protein and mRNA levels of ErbB3 in NPC tissues and adjacent normal tissues were detected by Western blotting and real-time quantitative PCR(qPCR),respectively.ErbB3 mRNA level and its correlation with the clinicopathological features and prognosis were analyzed.Results Results of Western blotting showed that the protein level of ErbB3 in 30 NPC tissues was higher than that in adjacent tissues(P<0.05).Results of qPCR showed that the mRNA level of ErbB3 in 65 NPC tissues was 4.625±1.980,higher than 1.033±0.600 in adjacent tissues(P<0.05).Univariate analysis showed that ErbB3 mRNA level was not related to gender,age,pathological classification,maximum tumor diameter and recurrence of NPC patients(P>0.05),but related to T stage of tumor invasion depth,N stage of lymph node metastasis,M stage of distant metastasis and comprehensive TNM stage(P<0.05).The median overall survival(OS)of ErbB3 mRNA high-level group and low-level group were 52.0 months and unreached with 3-year overall survival rates of 75.0%and 86.2%and 5-year overall survival rates were 33.3%and 65.5%(P<0.05).Multivariate analysis showed that ErbB3 mRNA level was an independent risk factor for OS in patients with NPC(HR=2.907,95%CI:1.372-6.160,P=0.005).Conclusion ErbB3 plays a crucial role in the occurrence and development of NPC and is closely related to the prognosis.ErbB3 may act as a new indicator for predicting the prognosis of NPC and a new target for clinical treatment.