LncRNA SNHG6在骨肉瘤患者血清中的表达及与临床病理特征的相关性

Expression of LncRNA SNHG6 in patients with osteosarcoma and its correlation with clinicopathological characteristics

目的探讨长链非编码RNA小核仁RNA宿主基因6在骨肉瘤患者血清中的表达及与临床病理特征的相关性。方法将128例疑似骨肉瘤患者以病理诊断结果分为骨肉瘤组62例与非骨肉瘤组66例,选取同期健康体检者135名设为健康组。比较3组血清长链非编码RNA小核仁RNA宿主基因6相对表达量,比较骨肉瘤组癌组织细胞、癌旁组织细胞长链非编码RNA小核仁RNA宿主基因6相对表达量,分析骨肉瘤患者血清长链非编码RNA小核仁RNA宿主基因6表达与临床特征的相关性,分析血清长链非编码RNA小核仁RNA宿主基因6对骨肉瘤的诊断价值。结果骨肉瘤组与非骨肉瘤组血清长链非编码RNA小核仁RNA宿主基因6相对表达量显著高于健康组(P<0.01),骨肉瘤组血清长链非编码RNA小核仁RNA宿主基因6相对表达量显著高于非骨肉瘤组(P<0.01)。骨肉瘤组癌组织细胞长链非编码RNA小核仁RNA宿主基因6相对表达量显著高于癌旁组织(P<0.01)。发生肺部转移、病理低分化、临床Ⅲ期~Ⅳ期者长链非编码RNA小核仁RNA宿主基因6相对表达量显著高于无肺部转移、病理中高分化、临床Ⅰ期~Ⅱ期者(P<0.05或0.01)。血清长链非编码RNA小核仁RNA宿主基因6预测骨肉瘤的截断值、灵敏度、特异度、准确度、曲线下面积分别为1.95、85.48%、56.06%、70.31%、0.801。结论血清长链非编码RNA小核仁RNA宿主基因6的表达在骨肉瘤组织内明显上调,与临床分期、病理分化、肺部转移有关,可作为临床骨肉瘤诊断及病情判断的潜在生物学标志物。

Objective To explore the expression of long non-coding RNA small nucleolar RNA host gene 6(LncRNA SNHG6) in patients with osteosarcoma and its correlation with clinicopathological characteristics. Methods A total of 128 patients with suspected osteosarcoma were divided into osteosarcoma group(62 cases) and non-osteosarcoma group(66 cases) based on the results of pathological diagnosis, and 135 healthy subjects during the same period were set as the healthy group. The relative expression of serum LncRNA SNHG6 in the three groups was compared, and the relative expression of LncRNA SNHG6 in cancer tissue cells and adjacent tissue cells of osteosarcoma group was compared. The correlation between the expression of serum LncRNA SNHG6 and clinical characteristics in patients with osteosarcoma was analyzed, and the diagnostic value of serum LncRNA SNHG6 in osteosarcoma was analyzed. Results Compared with the healthy group, the relative expression of serum LncRNA SNHG6 in the osteosarcoma group and the non-osteosarcoma group was significantly higher(P<0.01). Compared with the non-osteosarcoma group, the relative expression of serum LncRNA SNHG6 in the osteosarcoma group was significantly higher(P<0.01). The relative expression of LncRNA SNHG6 in cancer tissue cells in osteosarcoma group was significantly higher than that in adjacent tissue(P<0.01). The relative expression of LncRNA SNHG6 in patients with pulmonary metastasis, pathologically poorly differentiated patients, and clinical stage Ⅲ-Ⅳ patients was higher than that in patients without pulmonary metastasis,pathologically well differentiated patients,and clinical stage Ⅰ-Ⅱ patients(P<0.05).The best cut-off point,sensitivity,specificity,accuracy and area under the curve(AUC)of serum LncRNA SNHG6in predicting osteosarcoma were 1.95,85.48%,56.06%,70.31%and 0.801,respectively.Conclusions The expression of serum LncRNA SNHG6is significantly up-regulated in patients with osteosarcoma,which is related to clinical stage,pathological differentiation and lung metastasis.It can be used as a potential biomarker for clinical diagnosis and condition judgment of osteosarcoma.