超高效液相色谱-串联质谱法测定毛发中5种芬太尼类新精神活性物质的方法研究与应用

Establishment and Application of UPLC-MS/MS Method for 5 Fentanyl-Analogs of New Psychoactive Substance in Human Hair

本文建立同时分析毛发中5种芬太尼类新精神活性物质的超高效液相色谱-串联质谱(UPLC-MS/MS)测定方法.该方法以外标法定量,毛发样品经甲醇超声提取后用HSS T3 C18色谱柱分离,采用多反应监测模式(MRM)测定目标物。5种芬太尼类物质在质量浓度0.1~50 ng/mL范围内,呈现良好的线性关系且相关系数大于0.99,5种芬太尼类物质的检出限为0.05 ng/mg,定量限为0.1 ng/mg,日内和日间变异系数均小于15%,方法回收率在90.5%~108.5%之间。运用该方法检测添加样品,能有效检测目标物且回收率较好。该方法操作简单,灵敏度高,选择性好,可用于毛发中芬太尼类物质的分析测定。

To establish an ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)rapid determination method for simultaneous analysis of 5 fentanyl-analogs in human hair.Quantifies by external standard method,the hair was extracted with methanol by ultrasonic extraction and separated on Waters HSS T3 C18 column.Then target objects were simultaneously analyzed with multiple reaction monitoring(MRM)mode.5 fentanyl-analogs had a good linear relationship within the range of 0.1-50 ng/mL,and correlation coefficients were larger than 0.99.The limits of detection(LOD)of 5 fentanyl-analogs were 0.05 ng/mg,and the limits of quantitation(LOQ)were 0.1 ng/mg.The coefficient of variation were all less than 15%.The recovery of the method was 90.5%-108.5%.Apply this method to test spiked samples,The results showed all compounds were detected and had good recovery.The established method has the advantages of simple pretreatment,high sensitivity and good selectivity,the method can be used for the determination of fentanyl-analogs in human hair analysis.