液相色谱-串联质谱法检测一起误食曼陀罗引起的食物中毒物质

Identify the toxicological factors of a food poisoning caused by ingestion of Datura stramonium L.using liquid chromatography-tandem mass spectrometry

目的对一起疑似食用野菜中毒事件进行实验室检测分析,为中毒事件的处置和患者救治提供科学依据。方法利用液相色谱-三重四级杆串联质谱(LC-MS/MS)对采集的样本进行阿托品、东莨菪碱、山莨菪碱定性和定量检测。对现场采集的可疑植物进行形态学鉴定,结合流行病学调查确定中毒因子。结果从中毒当日患者食用的野菜饼和医院提供的生物样本中检出阿托品、东莨菪碱、山莨菪碱等莨菪烷类生物碱,在现场采集到的有毒植物经形态学鉴定为曼陀罗(Datura stramonium L.),患者临床症状及潜伏期与曼陀罗中毒表现相类似。结论本次事件是由误食曼陀罗引起的食物中毒,实验室检测结果为中毒事件快速、准确的确证起到了关键作用。

Objective To analyze a suspected poisoning event of edible wild vegetables by laboratory analysis,and provide scientific basis for the treatment of poisoning event and the treatment of patients.Methods The samples were qualitatively and quantitatively determined for atropine,scopolamine and anisodamine by liquid chromatography-triple quadrupole tandem mass spectrometry(LC-MS/MS).Morphological identification was carried out on the suspicious plants collected on site,and the poisoning factors were determined by epidemiological investigation.Results On the day of poisoning,tropane alkaloids,such as atropine,scopolamine and anisodamine,were detected in the wild vegetable cake and biological samples provided by the hospital.The poisonous plant was identified as Datura stramonium L using morphological analysis.The clinical symptoms and incubation period of the patient were similar to the poisoning performance of Datura stramonium L.Conclusion The food poisoning was caused by Datura stramonium L,the laboratory test results played a key role in the rapid and accurate identification of the poisoning event.