2种中药免疫增强剂对肉仔鸡新城疫疫苗免疫效果的影响

Effects of Two Traditional Chinese Medicine Immune Enhancers on Immune Efficacy of Newcastle Disease Vaccine of Broiler Chicks

[目的]探究2种中药免疫增强剂对肉仔鸡新城疫疫苗免疫效果的影响。[方法]将150只1日龄AA肉仔公鸡随机分为对照(CG)组、黄芪(AM)组和枸杞(LB)组,每组50只。CG组饲喂基础日粮,AM组和LB组在基础日粮中分别添加1%AM粉和1%LB粉,分别于14和35日龄测定免疫器官指数,14、21、28、35日龄测定新城疫病毒抗体(NDV-Ab)效价,14和28日龄测定血清免疫球蛋白含量。[结果]14日龄时,AM组脾脏指数(SpI)和法氏囊指数(BFI)较CG组分别提高31.17%(P<0.01)和34.86%(P<0.05);AM组SpI较LB组提高14.12%(P<0.05);35日龄时,LB组胸腺指数(ThI)和BFI较CG组分别提高14.29%(P<0.05)和9.76%(P<0.05),AM组ThI、SpI、BFI较CG组分别提高18.37%(P<0.05)、35.84%(P<0.05)、15.85%(P<0.05),AM组SpI较LB组提高19.92%(P<0.05)。与CG组相比,LB组NDV-Ab效价在28日龄提高34.78%(P<0.05),AM组NDV-Ab效价在14、21、28 d日龄分别提高118.18%(P<0.05)、75.00%(P<0.05)、56.52%(P<0.05)。与CG组相比,AM组血清免疫球蛋白M(IgM)含量在14和28日龄分别提高15.15%(P<0.05)和11.76%(P<0.05),血清免疫球蛋白G(IgG)含量分别提高7.50%(P<0.05)和14.15%(P<0.05);LB组血清IgM含量在14日龄较CG组提高12.12%(P<0.05),血清IgG含量在28日龄提高11.32%(P<0.05)。[结论]日粮中添加1%AM粉或1%LB粉可提高AA肉仔鸡主要免疫器官指数、NDV-Ab效价以及血清IgG、IgM含量,以添加1%AM粉效果较好。

[Objective]To assess the effects of two traditional Chinese medicine immune enhancers on immunological response of broiler chicks to Newcastle disease vaccine.[Method]In total,1501-day-old male AA broiler chicks were randomly allocated into three groups,a control(CG)group,a Astragalus membranaceus(AM)group,and a Lycium barbarum(LB)group,with 50 individuals in each group.The CG group fed basal diet,while the AM and LB groups were supplemented with 1%AM powder and 1%LB powder in basal diet,respectively.At 14 and 35 days of age,the immune organ indexes were measured.The Newcastle disease virus antibody(NDV-Ab)titer was determined at 14,21,28 and 35 days of age,and the contents of serum immunoglobulin were evaluated at 14 and 28 days of age.[Result]At 14 days of age,the spleen index(SpI)and bursa Fabricius index(BFI)in the AM group were 31.17%(P<0.01)and 34.86%(P<0.05)higher than those in the CG group,respectively,and the SpI in the AM group was 14.12%(P<0.05)higher than that in the LB group.At 35 days of age,the thymus index(ThI)and BFI in the LB group were 14.29%(P<0.05)and 9.76%(P<0.05)higher than those in the CG group,respectively;the ThI,SpI and BFI in the AM group were 18.37%(P<0.05),35.84%(P<0.05)and 15.85%(P<0.05)higher than those in the CG group,respectively;the SpI in the AM group was 19.92%(P<0.05)higher than that in the LB group.Compared with the CG group,the NDV-Ab titer of the LB group was increased by 34.78%(P<0.05)at 28 days of age,while that of the AM group was increased by 118.18%(P<0.05),75.00%(P<0.05)and 56.52%(P<0.05)at 14,21 and 28 days of age,respectively.At 14 and 28 days of age,compared with the CG group,the content of serum immunoglobulin M(IgM)in the AM group was increased by 15.15%(P<0.05)and 11.76%(P<0.05),respectively,and that of serum immunoglobulin G(IgG)was increased by 7.50%(P<0.05)and 14.15%(P<0.05),respectively.In comparison to the CG group,the content of serum IgM in the LB group was 12.12%(P<0.05)higher at 14 days of age,and that of serum IgG was 11.32%(P<0.05)higher at 28 days of age.[Conclusion]Dietary supplementation of 1%AM powder or 1%LB powder improved the major immune organ indexes,NDV-Ab titer,and serum IgG and IgM contents of AA broiler chicks.The effects of 1%AM powder was superior.