舟山地区抗ENA谱阳性关节炎患者的血清细胞因子谱分析

Multiplex cytokine analysis of patients with joint pain and positive extractable nuclear antigen in Zhoushan Area

目的分析本地区抗可溶性抗原(ENA)阳性的骨关节患者的血清细胞因子谱。方法选取2020年1月-12月在本地三甲医院经血清抗ENA谱检测至少有一项阳性的骨关节炎患者87例,其中男性11例,女性76例,平均年龄为(53.02±13.46)岁。从中选取10例患者与10例健康体检者用酶联免疫试验(ELISA)进行血清10项细胞因子筛查,筛选得到IFN-γ、IL-2、IL-4、IL-6进行分析。结果87例患者的抗ENA阳性指标多数以SSA和Ro-52为主。抗ENA谱阳性患者组的血清IFN-γ、IL-2、IL-4、IL-6均高于对照组,差异具有统计学意义(P<0.01)。抗ENA谱阳性项与IFN-γ、IL-2、IL-4、IL-6呈相关性,相关系数分别为0.587、0.403、0.480、0.376(P<0.01)。结论抗ENA阳性的骨关节患者血清细胞因子分析有助于自身免疫性疾病的诊疗。

Objective To perform a multiplex cytokine analysis of patients with joint pain and positive extractable nuclear antigen(ENA)in Zhoushan Area.Methods From January to December 2020,87 patients with joint pain and positive ENA were selected,including 11 male and 76 female with an average age of 53.02±13.46.Ten patients and ten healthy subjects were selected for screening of 10 cytokines in serum by enzyme-linked immunosorbent assay(ELISA),and IFN-γ,IL-2,IL-4 and IL-6 were screened for analysis.Results The positive index of ENA were mainly composed of SSA and Ro-52.Serum levels of IFN-γ,IL-2,IL-4,IL-6 in 87 patients were significantly higher than the control group,with the differences statistically significant(P<0.01).There was correlation between positive ENA items and IFN-γ,IL-2,IL-4,IL-6,and the coefficients were 0.587,0.403,0.480,0.376(P<0.01).Conclusion Serum cytokine analysis of patients with joint pain and positive ENA is helpful for the diagnosis and treatment of autoimmune diseases.