不同运动方式对2型糖尿病小鼠肠道菌群及短链脂肪酸的影响

Effects of different exercise modes on intestinal flora and short-chain fatty acids in mice with type 2 diabetes

目的:探索有氧运动、抗阻运动及联合有氧—抗阻运动对2型糖尿病小鼠肠道菌群种类和丰度、短链脂肪酸(SCFA)及血糖的影响。方法:选用8周龄C57BL/KS.db背景的雄性db/db小鼠作为小鼠模型,将小鼠随机分为有氧运动组(Y组)、抗阻运动组(K组)、有氧联合抗阻运动组(L组)和对照组(D组),每组10只,经过8周的干预后,检测各组小鼠血糖、肠道菌群及粪便和血清中的SCFA含量。结果:运动干预8周后,K组、Y组、L组小鼠血糖均低于D组,肠道放线菌门相对丰度均高于D组,特有菌种数量均高于D组(均P<0.05)。K组2型糖尿病小鼠的chao 1指数和shannon指数高于D组,Y组chao 1指数高于D组,L组shannon指数高于D组(均P<0.05)。K组和L组粪便乙酸含量高于D组和Y组,K组粪便丙酸含量高于D组、Y组、L组(P<0.05);各运动组粪便丁酸含量均高于D组(均P<0.05),K组、L组血清丁酸含量均高于D组(P<0.05)。抗阻运动干预的2型糖尿病小鼠罗氏菌属和瘤胃球菌属相对丰度更高。结论:有氧运动、抗阻运动和联合有氧—抗阻运动均可改善2型糖尿病小鼠的血糖及肠道菌群的组成和结构,提高SCFA的水平,且抗阻运动在改善肠道菌群结构及提高SCFA水平上的效果最佳。

Objective:To explore the effects of aerobic exercise,resistance exercise and combined aerobic-resistance exercise on the intestinal flora species and abundance,short-chain fatty acids(SCFA)and blood glucose in type 2 diabetic mice.Methods:Eight-week-old male db/db mice of C57BL/KS.db background were used as mouse models,and the mice were randomly divided into aerobic exercise group(Group Y),resistance exercise group(Group K),combined aerobic-resistance exercise group(Group L)and control group(Group D),10 mice each.After 8 weeks of intervention,blood glucose,intestinal flora and SCFA content in feces and serum were measured.Results:After 8 weeks of exercise intervention,the blood glucose of group K,group Y and group L was lower than that of group D,the relative abundance of intestinal actinomycetes was higher than that of group D(P<0.05),and the number of specific species was higher than that of group D.The chao 1 index and shannon index of mice with type 2 diabetes in group K were higher than those of group D,the chao 1 index of group Y was higher than that of group D,and the shannon index of group L was higher than that of group D(P<0.05).The fecal acetic acid content was higher in groups K and L than that in groups D and Y,and the fecal propionic acid content was higher in group K than that in groups D,Y and L(P<0.05).The fecal butyric acid content was higher in all exercise groups than that in group D,and the serum butyric acid content was higher in groups K and L than that in group D(P<0.05).The relative abundance of Rhothia and Ruminococcus was higher in type 2 diabetic mice receiving resistance exercise intervention.Conclusion:Aerobic exercise,resistance exercise and combined aerobic-resistance exercise all improved the blood glucose and the composition and structure of intestinal flora and increased the level of SCFA in type 2 diabetic mice,and resistance exercise was the most effective in improving the structure of intestinal flora and increasing the level of SCFA.