lncRNA MALAT1和miR-203在慢性心力衰竭患者中的表达水平及临床意义

Expression levels and clinical significance of lncRNA MALAT1 and miR-203 in patients with chronic heart failure

目的 探讨慢性心力衰竭(CHF)患者血清长链非编码RNA(lncRNA)肺腺癌转录子1(MALAT1)和微小RNA-203(miR-203)水平及临床意义。方法 选取湖北省宜昌市第二人民医院/三峡大学第二人民医院2018年5月至2020年2月收治的68例CHF患者作为CHF组,另选取70例同期体检健康者作为对照组,收集两组一般资料及患者随访1年不良预后发生情况,采用实时荧光定量PCR(RT-qPCR)法检测两组血清lncRNA MALAT1、miR-203水平,Pearson法分析CHF患者血清lncRNA MALAT1、miR-203与左室射血分数(LVEF)、左室舒张末内径(LVEDD)的相关性,并分析血清lncRNA MALAT1、miR-203水平与CHF患者预后的关系。结果 CHF组LVEDD及lncRNA MALAT1水平高于对照组(P<0.05),LVEF及miR-203水平低于对照组(P<0.05);不同心功能分级患者血清lncRNA MALAT1及miR-203水平比较,差异有统计学意义(P<0.05)。CHF患者血清lncRNA MALAT1水平与LVEF呈负相关(r=-0.526,P<0.001),与LVEDD正相关(r=0.475,P<0.001);血清miR-203水平与LVEF呈正相关(r=0.481,P<0.001),与LVEDD负相关(r=-0.463,P<0.001)。lncRNA MALAT1高表达组不良预后发生率高于lncRNA MALAT1低表达组(P<0.05),miR-203低表达组不良预后发生率高于miR-203高表达组(P<0.05)。结论 CHF患者血清lncRNA MALAT1呈高表达,miR-203呈低表达,二者均与患者心功能及不良预后有关,可能成为CHF患者预后评估的潜在标志物。

Objective To investigate the levels of long non-coding RNA(lncRNA) metastasis-associated lung adenocarcinoma transcript 1(MALAT1) and microRNA-203(miR-203) in the serum of patients with chronic heart failure(CHF) and their clinical significance.Methods A total of 68 CHF patients admitted to the Second People′s Hospital of Yichang/the Second People′s Hospital of China Three Gorges University from May 2018 to February 2020 were selected as the CHF group,and 70 patients with healthy physical examination during the same period were selected as the control group.The general information of the two groups and the adverse prognosis of the patients after 1 year of follow-up were collected,the levels of serum lncRNA MALAT1 and miR-203 in the two groups were detected with RT-qPCR method,the correlation between serum lncRNA MALAT1,miR-203 and left ventricular ejection fraction(LVEF) and left ventricular end diastolic diameter(LVEDD) in CHF patients was analyzed with Pearson method,and the relationship between serum lncRNA MALAT1,miR-203 levels and the prognosis of CHF patients was analyzed.Results The LVEDD and level of lncRNA MALAT1 in the CHF group were higher than those in the control group(P<0.05),and the LVEF and level of miR-203 were lower than those in the control group(P<0.05);there was a statistically significant difference in serum lncRNA MALAT1 and miR-203 levels in patients with different cardiac function classes(P<0.05).The level of serum lncRNA MALAT1 in CHF patients negatively correlated with LVEF(r=-0.526,P<0.001) and positively correlated with LVEDD(r=0.475,P<0.001),the level of serum miR-203 positively correlated with LVEF(r=0.481,P<0.001) and negatively correlated with LVEDD(r=-0.463,P<0.001).The incidence of poor prognosis in the lncRNA MALAT1 high expression group was higher than that in the lncRNA MALAT1 low expression group(P<0.05),and the incidence of poor prognosis in the miR-203 low expression group was higher than that in the miR-203 high expression group(P<0.05).Conclusion Serum lncRNA MALAT1 is high in CHF patients,and miR-203 is low.The levels of both are related to the heart function and poor prognosis of patients,and may become potential markers for prognostic evaluation of CHF patients.