SFRP1和β-catenin在食管癌中的作用及相关机制

Role of SFRP1 and β-catenin in esophageal carcinoma and its related mechanism

目的 探讨分泌型卷曲相关蛋白1(SFRP1)和β-连环蛋白(β-catenin)在食管癌中的作用及相关机制。方法 选取该院肿瘤科2014年1月至2018年1月收治的食管癌患者121例作为研究对象,采集所有受试者癌组织及癌旁组织标本。采用甲基化特异性PCR(MSP)检测癌组织及癌旁组织SFRP1水平,采用免疫组织化学法检测癌组织及癌旁组织β-catenin水平。结果 食管癌患者癌组织SFRP1表达水平较癌旁组织均明显降低,β-catenin表达水平较癌旁组织明显升高,差异有统计学意义(P<0.05);是否淋巴结转移食管癌患者SFRP1表达阳性率差异有统计学意义(P<0.05),β-catenin表达阳性率差异无统计学意义(P>0.05);不同病理类型、分化程度、TNM分期食管癌患者SFRP1、β-catenin表达阳性率比较,差异有统计学意义(P<0.05)。结论 食管癌患者的癌组织中SFRP1表达下调,β-catenin表达上调,SFRP1、β-catenin表达水平与食管癌患者病情严重程度有关,可作为食管癌的早期标志物。

Objective To investigate role of secreted frizzled-related protein 1(SFRP1) and β-catenin in esophageal carcinoma its related mechanism.Methods A total of 121 patients with esophageal cancer admitted to the Department of Oncology of this Hospital from January 2014 to January 2018 were selected as the study subjects,and cancer tissue and adjacent tissue samples of all subjects were collected.Methylation specific PCR(MSP) was used to detect the level of SFRP1 in cancer tissues and adjacent tissues,and immunohistochemistry(IHC) was used to detect the level of β-catenin in cancer tissues and adjacent tissues.Results The expression level of SFRP1 in esophageal cancer tissues was significantly lower than that in adjacent tissues,and the expression level of β-catenin was significantly higher than that in adjacent tissues,with statistical significance(P<0.05).There was statistically significant difference in the positive rate of SFRP1 expression in esophageal cancer patients with lymph node metastasis(P<0.05),but no significant difference in β-catenin expression(P>0.05).There was significant difference in the positive rate of SFRP1 in esophageal cancer patients with different pathological types,degree of differentiation and TNM stage(P<0.05).Conclusion The expression levels of SFRP1 and β-catenin are down-regulated and up-regulated in esophageal cancer tissues.The expression levels of SFRP1 and β-catenin are related to the severity of esophageal cancer,which could be used as early markers of esophageal cancer.