滤纸法在三种温度条件下保存标准菌株应用初探

Application of filter paper method to preserve standard strains under three temperature conditions

目的 分析3种不同温度条件下滤纸法保存的标准菌株存活率、生物学性状和药敏试验结果的变化情况,以选择经济、有效的标准菌株保存方式。方法 用滤纸法将标准菌株于0℃、-20℃、-80℃3种温度条件下保存,在保存了1、2、3、7年后进行细菌的复苏,观察细菌的存活率、生物学性状,并用PhoenixM50全自动细菌鉴定及药敏分析系统进行细菌鉴定和药敏试验,观察标准菌株的基本生物学性状和药敏试验结果变化情况。结果 使用滤纸法保存的4种标准菌株,在0℃条件下3年存活率为100%,细菌基本生物学性状和药敏试验结果3年无明显变化;在-20℃、-80℃温度条件下,其7年存活率均为100%,细菌基本生物学性状和药敏试验结果也无明显变化。结论 滤纸法保存的非苛养类标准菌株在0℃条件下可短期(3年)保存,在-20℃、-80℃条件下可较长期(7年)保存。

Objective To analyze the changes of survival rate,biological characteristics and drug sensitivity test result of the standard strain under three different temperature conditions in order to select an economical and effective standard strain preservation method.Methods The standard strains were preserved at 0 ℃,-20 ℃ and-80 ℃ by filter paper method.After being preserved for 1,2,3 and 7 years,the bacteria were resuscitated to observe the survival rate and biological characteristics.The PhoenixM50 automatic bacterial identification and sensitivity analysis system were used for bacterial identification and drug sensitivity test result to observe the change of the basic biological characteristics and drug sensitivity test.Results The survival rate of the four standard strains preserved by filter paper method was 100% in 3 years at 0 ℃,and the basic biological characteristics and drug sensitivity test result of the bacteria had no obvious change in 3 years.Both of the survival rate were 100% at-20 ℃ and-80 ℃,and the basic biological characteristics and drug sensitivity test result of bacteria had no significant change in 7 years.Conclusion The non-fastidious standard strain preserved by filter paper method could be stored for a short period(3 years) at 0 ℃,and for a long period(7 years) at-20 ℃ and-80 ℃.