口腔扁平苔藓患者血清NGF、sPD-1、RANTES 水平与免疫功能的相关性分析

Correlation between serum NGF,sPD-1,RANTES levels and immune function in patients with oral lichen planus

目的探讨口腔扁平苔藓(OLP)患者血清神经生长因子(NGF)、可溶性程序性死亡受体1(sPD-1)、调节活化正常T细胞表达和分泌因子(RANTES)水平与免疫功能的相关性。方法将2018年3月至2020年3月陕西中医药大学第二附属医院收治的75例OLP患者纳入研究,按照病情严重程度分作糜烂型组(40例)及非糜烂型组(35例)。另选取同期于该院进行体检的健康志愿者35例作为对照组。检测并比较3组血清NGF、sPD-1及RANTES水平,以及外周血T淋巴细胞亚群和体液免疫指标水平。采用Pearson相关分析OLP患者血清NGF、sPD-1、RANTES水平与外周血T淋巴细胞亚群和体液免疫指标的相关性。结果糜烂型组和非糜烂型组的血清NGF、sPD-1、RANTES、免疫球蛋白(Ig)M水平及CD8^(+)均明显高于对照组,且糜烂型组高于非糜烂型组(均P<0.05);糜烂型组和非糜烂型组的CD3^(+)、CD4^(+)均低于对照组,且糜烂型组低于非糜烂型组(均P<0.05)。Pearson相关分析显示,OLP患者血清NGF、sPD-1、RANTES水平与CD3^(+)、CD4^(+)均呈负相关,与CD8^(+)、IgM水平均呈正相关(均P<0.05)。结论OLP患者血清NGF、sPD-1、RANTES水平均明显升高,且与患者细胞免疫及体液免疫密切相关。

Objective To investigate the correlation between serum nerve growth factor(NGF),soluble programmed death receptor 1(sPD-1),regulation-activated normal T cell expression and secreted factor(RANTES) levels and immune function in oral lich planus(OLP) patients.Methods A total of 75 OLP patients admitted to the Second Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine from March 2018 to March 2020 were included in this study,and they were divided into erosion group(40 cases) and non-erosion group(35 cases) according to the severity of the disease type.In addition,another 35 healthy volunteers who underwent physical examination in this hospital were selected as the control group.The serum NGF,sPD-1 and RANTES levels in the three groups were detected and compared,as well as the the peripheral blood T lymphocyte subsets and humoral immunity indexes.Pearson correlation analysis was used to analyze the correlation between serum NGF,sPD-1,RANTES levels and peripheral blood lymphocyte subsets and humoral immunity indexes in OLP patients.Results The serum NGF,sPD-1,RANTES,immunoglobulin(Ig) M levels and CD8^(+) in erosion group and non-erosion group were significantly higher than those in control group,and those in erosion group were significantly higher than those in non-erosion group(all P<0.05).The CD3^(+)、CD4^(+) in erosion group and non-erosion group were lower than those in control group,and those in erosion group were lower than those in non-erosion group(all P<0.05).Pearson correlation analysis showed that serum NGF,SPD-1 and RANTES levels in OLP patients were negatively correlated with CD3^(+) and CD4^(+),but positively correlated with CD8^(+) and IgM levels(all P<0.05).Conclusion The serum NGF,sPD-1 and RANTES levels in OLP patients significantly increased,which closely related to cellular immunity and humoral immunity.