马铃薯与枸杞嫁接愈合过程的解剖学观察

Anatomical Observation on the Graft Union Between Potato and Wolfberry

为揭示马铃薯与枸杞嫁接愈合过程,采用石蜡切片技术对马铃薯/枸杞嫁接植株进行解剖学观察。结果显示:马铃薯/枸杞嫁接植株生长旺盛,可完成整个生育进程。马铃薯/枸杞嫁接愈合过程可分为4个阶段:(1)隔离层产生期,嫁接5 d后,嫁接接口处产生隔离层;(2)愈伤组织形成期,嫁接9 d后,隔离层细胞脱分化,最后形成愈伤组织;(3)愈伤组织分化期,嫁接13 d后,愈伤组织分化,形成愈伤组织桥;(4)新的维管组织形成期,嫁接17 d后,形成新的韧皮部和木质部及维管束桥,使砧木和接穗完全结合,愈合过程结束。

In order to reveal the graft union process between potato and wolfberry,anatomical observation of potato/wolfberry grafting system was carried out using paraffin section technique.The results showed that the potato/wolfberry grafting system grew vigorously and could complete the entire reproductive process.The union process of potato/wolfberry grafting could be divided into four stages:(1)isolation layer generation period,5 d after grafting,an isolation layer was formed at the grafting interface;(2)callus formation period,9 d after grafting,the isolation layer cells dedifferentiated,finally callus was formed;(3)callus differentiation stage,13 d after grafting,the callus differentiated,forming callus bridge;(4)new vascular tissue formation period,17 d after grafting,new phloem,xylem and vascular bundle bridges were formed which made the rootstock and scion fully combined,and the union process was finished.