鸢尾草和体外冲击波对去势大鼠骨折愈合的影响

Effects of irisin combined with extracorporeal shock wave on fracture healing in ovariectomized rats

[目的]探究鸢尾素联合体外冲击波(extracorporeal shock waves,ESW)治疗去势大鼠骨质疏松性骨折的作用与机理.[方法]80只SD大鼠随机分为5组,每组16只.分别为假手术组、模型组、模型+ESW组、模型+鸢尾素组、模型+ESW+鸢尾素组.除假手术组仅行手术切口外,其他各组先后行双侧卵巢切除和左侧股骨骨折内固定,术后分别给予相应处理,其中,ESW治疗每周1次,鸢尾素组骨折部位局部注射.治疗后8周处死动物,行骨密度、破骨细胞指数、micro-CT、Western blot和RT-qPCR检测.[结果]在骨密度和破骨细胞指数均由优至劣低依次为,假手术组>模型+ESW+鸢尾素>模型+ESW组,或模型+鸢尾素组>模型组(P<0.05).MicroCT检测,包括BS/BV、Tb.N、Tb.Th和Tb.Sp,由优至劣低依次为,假手术组>模型+ESW+鸢尾素>模型+ESW组,或模型+鸢尾素组>模型组(P<0.05).Western blot检测BMP2、VEGF、Nrf2F的OH-1的蛋白相对表达量由高至低依次为,假手术组>模型+ESW+鸢尾素>模型+鸢尾素组,或模型+ESW组>模型组(P<0.05).RT-qPCR检测Nrf2和OH-1mRNA相对表达量由高至低依次为,假手术组>模型+ESW+鸢尾素>模型+鸢尾素组,或模型+ESW组>模型组(P<0.05).[结论]鸢尾素联合ESW可激活Nrf2/OH-1通路并促进VEGF和BMP2蛋白的表达,促进骨质疏松大鼠的骨折愈合.

[Objective]To explore the effect on mechanism of irisin combined with extracorporeal shock wave(ESW)on fracture healing in the ovariectomized rats.[Methods]A total of 80 SD rats were randomly divided into 5 groups with 16 rats in each group,including sham operation group,model group,model+ESW group,model+irisin group,model+ESW+irisin group.Except the animals in sham operation group that received surgical incision only,all rates received bilateral ovariectomy,and then left femoral fracture and internal fixation successively,subsequently,corresponding postoperative treatments were given respectively,including ESW treatment once a week,and local injection of irisin group in fracture site.At 4 weeks after treatment,the animals were sacrificed for bone mineral density,osteoclast index,microCT,Western blot and RT-QPCR assays.[Results]Bone mineral density and osteoclast index were ranked superior-inferiorly in orders of sham operation group>model+ESW+irisin group>model+ESW group or model+irisin group>model group(P>0.05).MicroCT detection items,including BS/BV,Tb.N,Tb.Th and Tb also ranked superior-inferiorly in sequence of model+ESW+irisin group>model+ESW group or model+irisin group>model group(P<0.05).In term of Western blot detection,the relative protein expression of BMP2,VEGF,Nrf2F and OH-1 arranged up-down in order of sham operation group>model+ESW+irisin group>model+ESW group or model+irisin group>model group(P<0.05).In term of RT-QPCR assay,the relative mRNA expression of Nrf2 and OH-1 from high to low were:sham operation group>model+ESW+irisin group>model+irisin group or model+ESW group>model group(P<0.05).[Conclusion]Irisin combined with ESW might activate Nrf2/OH-1 pathway to promote the expression of VEGF and BMP2 proteins,and promote fracture healing in the ovariectomized rats.