小麦麸皮硒化多糖的肝保护活性研究

Hepatoprotective activities of wheat bran Se-polysaccharide

为了探讨硒化改性对小麦麸皮多糖生理活性的影响,文章以麦麸为原料,采用超声波辅助热水提取法和DEAE纤维素-52层析法分离得到一多糖组分,命名为W2。利用HNO_(3)-Na_(2)SeO_(3)法改性获得富硒产物SeW2-3,并研究其对CCl_(4)诱导的小鼠急性肝损伤的保护活性。结果表明:与天然的小麦麸皮多糖相比,小麦麸皮硒化多糖SeW2-3具有更强的保肝活性;与模型组相比,硒化多糖组小鼠血清中天冬氨酸转氨酶(AST)和丙氨酸转氨酶(ALT)活性显著降低,肝脏中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性显著增加,且丙二醛(MDA)的量显著下降;与模型组相比,硒化多糖组小鼠血清中的炎性因子肿瘤坏死因子-α(TNF-α)和白介素-1β(IL-1β)水平显著降低,且肝脏中Caspase-3和Caspase-8相对活性显著降低。该文结果表明SeW2-3具有显著的肝保护活性。

In this study,in order to better understand the effects of selenium modification on the physiological activity of wheat bran polysaccharide,a polysaccharide fraction named W2 was isolated from wheat bran by ultrasound assisted hot water extraction and DEAE Cellulose-52 chromatography successively.The Se-enriched product SeW2-3 was obtained by using HNO_(3)-Na_(2)SeO_(3) method,and its hepatoprotective effect on CCl_(4)-induced mice acute liver injury was investigated.The results showed that compared with natural wheat bran polysaccharide,wheat bran Se-polysaccharide SeW2-3 has a stronger hepatoprotective activity.Compared with the model control group,in the Se-polysaccharide groups,the activities of the aspartate aminotransferase(AST)and alanine aminotransferase(ALT)in the serum of mice significantly decreased,the activities of the superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and catalase(CAT)in the liver were significantly enhanced,and the content of malondialdehyde(MDA)decreased.In addition,in the Se-polysaccharide groups,the levels of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)in mice serum significantly decreased,and the relative activities of Caspase-3 and Caspase-8 in the liver significantly decreased.These results indicate that SeW2-3 has significant hepatoprotective activity.