老年性白内障患者晶状体上皮细胞CNN3、YAP表达与晶状体上皮细胞凋亡的相关性研究

Correlation between the expression of CNN3 and YAP in lens epithelial cells of patients with senile cataract and the apoptosis of lens epithelial cells

目的 探讨老年性白内障患者晶状体上皮细胞中钙调节蛋白3(CNN3)、Yes相关蛋白(YAP)信使核糖核酸(mRNA)和蛋白表达水平与晶状体上皮细胞凋亡的关系。方法 选择2019年2月至2021年9月该院收治的67例白内障患者作为老年性白内障组,另选取同期于该院进行角膜移植术并排除白内障的42例患者作为对照组。手术获得患者晶状体前囊膜(晶状体前囊膜主要由上皮细胞构成),检测晶状体上皮细胞中CNN3、YAP mRNA和蛋白表达水平及晶状体上皮细胞凋亡率,分析老年性白内障患者晶状体上皮细胞中CNN3 mRNA与YAP mRNA表达水平的相关性及CNN3 mRNA、YAP mRNA与细胞凋亡率的相关性。结果 与对照组比较,老年性白内障组晶状体上皮细胞中CNN3 mRNA和蛋白表达水平升高,YAP mRNA和蛋白表达水平降低(P<0.05);老年性白内障患者晶状体上皮细胞中CNN3 mRNA与YAP mRNA表达水平呈负相关(P<0.05);老年性白内障组晶状体上皮细胞凋亡率高于对照组(P<0.05);老年性白内障患者晶状体上皮细胞中CNN3 mRNA表达水平与晶状体上皮细胞凋亡率呈正相关(P<0.05),YAP mRNA表达水平与晶状体上皮细胞凋亡率呈负相关(P<0.05)。结论 老年性白内障患者晶状体上皮细胞中CNN3呈高表达、YAP呈低表达,二者与晶状体上皮细胞凋亡率有关,可能参与老年性白内障疾病的发生。

Objective To investigate the relationship between the expression levels of calmodulin 3(CNN3),Yes-associated protein(YAP)messenger ribonucleic acid(mRNA)and protein in lens epithelial cells of patients with senile cataract and lens epithelial cell apoptosis.Methods A total of 67 cataract patients admitted to this hospital from February 2019 to September 2021 were selected as the senile cataract group,and 42 patients who underwent corneal transplantation in the hospital during the same period and had cataracts excluded were selected as the control group.The anterior lens capsule of the patient was obtained by surgery(the anterior lens capsule was mainly composed of epithelial cells),and the expression levels of CNN3 and YAP mRNA and protein in lens epithelial cells and the apoptosis rate of lens epithelial cells were detected.The correlation between the expression levels of CNN3 mRNA and YAP mRNA in lens epithelial cells of patients with senile cataract and the correlation between CNN3 mRNA and YAP mRNA and the apoptosis rate were analyzed.Results Compared with the control group,the expression levels of CNN3 mRNA and protein in lens epithelial cells of the senile cataract group were increased,and the expression levels of YAP mRNA and protein were decreased(P<0.05).The apoptosis rate of lens epithelial cells in the senile cataract group was higher than that in the control group(P<0.05).The expression level of CNN3 mRNA in lens epithelial cells of patients with senile cataract was positively correlated with the apoptosis rate of lens epithelial cells(P<0.05),and the expression level of YAP mRNA was negatively correlated with the apoptosis rate of lens epithelial cells(P<0.05).Conclusion The high expression of CNN3 and the low expression of YAP in lens epithelial cells of senile cataract patients are related to the apoptosis rate of lens epithelial cells and may be involved in the occurrence of senile cataract disease.