LINC00668在肺鳞癌中高表达并促进肿瘤细胞的侵袭转移

LINC00668 is Highly Expressed in Lung Squamous Cell Carcinoma and Promotes Tumor Cell Migration and Invasion

背景与目的由于缺乏有效的治疗措施,肺鳞癌(lung squamous cell carcinoma,LUSC)仍是制约非小细胞肺癌(non-small cell lung cancer,NSCLC)5年生存率的重要影响因素。长链非编码RNA 00668(LINC00668)参与调控多种肿瘤的发生发展,但其在LUSC中的研究尚不完善。本研究旨在探讨LINC00668在NSCLC特别是LUSC中的预后价值及生物学功能。方法本研究利用癌症基因组图谱(The Cancer Genome Altas,TCGA)数据库分析了LINC00668在NSCLC特别是LUSC中的表达模式及其与患者临床特征和预后的关系;并通过体外实验探索了其在LUSC细胞中的功能。结果LINC00668在LUSC组织中高表达,并与患者的肿瘤原发灶-淋巴结-转移(tumor-nodemetastasis,TNM)分期显著相关。同时,LINC00668在吸烟患者中的表达量明显升高,并与吸烟LUSC患者的总生存期(overall survival,OS)显著相关。体外实验显示,相较于正常支气管上皮细胞和癌旁组织,LINC00668在LUSC细胞系和组织样本中表达量显著升高;同时,在LUSC细胞系中敲低LINC00668可有效抑制细胞的侵袭转移能力。结论LINC00668可能与肺鳞癌发生发展密切相关,抑制LINC00668可在一定程度上减少LUSC的转移。

Background and objective A lack of effective treatment for lung squamous cell carcinoma(LUSC)makes it an important factor restricting the 5-year survival rate of non-small cell lung cancer(NSCLC).Long non-coding RNA 00668(LINC00668)was reported to play crucial regulatory roles in the tumorigenesis and progression of various cancers;however,its role in LUSC is unclear.The aim of this study was to investigate the prognosis value and biological function of LINC00668 in NSCLC,especially in LUSC.Methods The expression pattern of LINC00668 and its relationship with clinical characteristics and prognosis of patients were investigated in the NSCLC especially LUSC based on The Cancer Genome Altas(TCGA)database.Its function in LUSC cells was explored in vitro.Results LINC00668 expression was significantly up-regulated in LUSC patients and high expression level of LINC00668 was associated with advanced tumor-node-metastasis(TMN)stage.Moreover,the expression of LINC00668 significantly increased in smoking patients,and was a prognostic indicator for overall survival(OS)of smoking patients with LUSC.In vitro experiments showed that LINC00668 has significantly higher expression level in LUSC cell lines and tissues compared to normal bronchial epithelial cell and para-tumor tissues;meanwhile,functional assay indicated knockdown of LINC00668 effectively inhibited the migration and invasion of LUSC cells.Conclusion LINC00668 might closely relate to the development of LUSC,and inhibition of LINC00668 may reduce the metastasis of LUSC.