清肺降霾汤对尿液中PM_(2.5)相关代谢物巯基尿酸含量的影响

Effect of Qingfei Jiangmai Decoction on Content of PM_(2.5)-related Metabolites Mercapturic Acids in Urine

目的:探索大气细颗粒物(PM_(2.5))污染期间中药复方清肺降霾汤对健康人群尿液中巯基尿酸含量的影响。方法:招募北京地区18至30岁健康医学生84例作为受试志愿者,按照随机对照原则分为观察组(42例,脱落1例)及对照组(42例,脱落3例),大气PM_(2.5)污染期间前后两批次分别给予清肺降霾汤配方颗粒及安慰剂颗粒连续服用7 d(1袋/次,2次/d),两批次间隔时间为4周。干预期间记录时间-活动模式。采用在线固相萃取二维液相色谱串联质谱技术(On-line SPE-LC-MS/MS)检测干预前后尿液中PM_(2.5)相关代谢产物包括苯巯基尿酸(SPMA)、N-乙酰基-S-(3-羟基丙基)半胱氨酸(3-HPMA)、N-乙酰基-S-(3-羟基丙基-1-甲基)半胱氨酸(HMPMA)、N-乙酰基-S-(2-腈基乙基)-L-半胱氨酸(CEMA)及N-乙酰基-S-(2-羟基乙基)-L-半胱氨酸(HEMA)的含量并进行统计分析。结果:与本组干预前比较,观察组干预后CEMA、HEMA、3-HPMA、HMPMA均高于干预前,其中HEMA差异具有统计学意义(P<0.05);与对照组干预后比较,观察组干预后HEMA、SPMA明显高于对照组(P<0.05),观察组HEMA(Z=-3.614,P<0.01)、HMPMA(Z=-1.988,P<0.05)干预前后差值明显大于对照组。控制干预前尿液浓度,干预后观察组HEMA显著高于对照组(F=7.597,P<0.01)。结论:大气PM_(2.5)污染期间,清肺降霾汤干预可显著增加北京地区健康人尿液中环氧乙烷代谢物HEMA的排泄,增强PM_(2.5)中有毒成分的脱毒过程,具有防霾治霾实际运用价值。

Objective:To explore the effect of Qingfei Jiangmai decoction(QJD)on the content of mercapturic acids in urine in healthy people amid PM_(2.5)(particles 2.5 microns or less in size)pollution.Method:A total of 84 healthy students of 18-30 years old in Beijing were recruited and they were randomized into the test group(42 in total,with 1 dropout)and control group(42 in total,with 3 dropouts).During the pollution,the test group and the control group respectively took QJD granules and placebo for 7 days(1 bag/time,2 times/day),and another 7-day intervention with the same drugs was performed at an interval of4 weeks.The time-activity patterns were recorded during the intervention.On-line solid phase extraction-liquid chromatography/tandem mass spectrometry(SPE-LC-MS/MS)was performed to detect the content of PM_(2.5)-related metabolites S-phenylmercapturic acid(SPMA),3-hydroxypropylmercapturic acid(3-HPMA),3-hydroxy-1-methylpropylmercapturic acid(HMPMA),N-acetyl-S-(2-nitrile ethyl)-L-cysteine(CEMA),and N-acetyl-S-(2-hydroxy ethyl)-L-cysteine(HEMA)in urine before and after intervention.Statistical analysis was followed.Result:The content of CEMA,HEMA,3-HPMA,and HMPMA in the test group was all higher after the intervention than before the intervention,with the significant difference in HEMA(P<0.05).After intervention,content of HEMA and SPMA was significantly higher in the test group than in the control group(P<0.05),and the difference in HEMA(Z=-3.614,P<0.01)and HMPMA(Z=-1.988,P<0.05)before and after invention in the test group was significantly larger than that in the control group.After the intervention,HEMA in the test group was significantly higher than that in the control group(F=7.597,P<0.01).Conclusion:During PM_(2.5)pollution,QJD can increase the excretion of HEMA,a metabolite of ethylene oxide,in the urine of healthy people in Beijing,and enhance the detoxification process of toxic components in PM_(2.5),which is of great value in preventing and treating haze-related illnesses.