内生真菌SH09对丹参生长和活性成分积累的影响

Effects of endophytic fungus SH09 on plant growth and accumulation of active components in Salvia miltiorrhiza

目的研究内生真菌Epichloëbromicola SH09是否对丹参的生长和有效成分积累有促进作用,以提高药用植物丹参的品质。方法制备E.bromicola SH09固体菌肥,与丹参苗共培养60 d和120 d,分别测定E.bromicola处理组和对照组丹参的4种形态指标、根的鲜重、根的干重及根中4种丹参酮含量和2种丹参酚酸含量。结果共培养60 d和120 d后,E.bromicola SH09可显著提高丹参苗分蘖数、株高、叶片数、叶面积、根鲜重、根干重及根中丹参酮和丹参酚酸的积累量。结论内生真菌E.bromicola SH09能有效促进丹参生长发育和活性成分积累。本研究结果不仅有助于拓宽内生真菌新生态功能的研究,同时有助于提高药用植物丹参的品质。

Objective To study the effects of endophytic fungus Epichloëbromicola SH09 on the plant growth and accumulation of active components in Salvia miltiorrhiza,and improve the quality of medicinal plant S.miltiorrhiza.Methods E.bromicola SH09 solid bacterial fertilizer was prepared and co-cultured with S.miltiorrhiza for 60 d and 120 d.Four morphological indexes,fresh weight of roots,dry weight of roots,and the contents of four tanshinones and two phenolic acids in the roots of S.miltiorrhiza from treated group and control group were assayed,respectively.Results After 60 d and 120 d co-culture,E.Bromicola SH09 significantly increased the tiller number,plant height,leaf number,leaf area,fresh weight of roots,dry weight of roots,and the content of tanshinones and phenolic acids in S.miltiorrhiz.Conclusion The endophytic fungus E.bromicola SH09 can effectively promote the plant growth and improve the accumulation of active components in S.miltiorrhiza,which not only broadens the new ecological functions of endophytic fungi,but also improves the quality of medicinal plant S.miltiorrhiza.