基于UHPLC-Q-Orbitrap HRMS的不同年限陈皮入血成分分析

Analysis of blood components of Citri Reticulatae Pericarpium in different years based on UHPLC-Q-Orbitrap HRMS

本研究拟应用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UHPLC-Q-Orbitrap HRMS)技术对不同年限广陈皮进行入血成分鉴定研究。课题组采用UHPLC-Q-Orbitrap HRMS技术,以及Compound Discover 3.0软件进行分析,比较广陈皮提取液、给药血浆、空白血浆的图谱差异,将分子量、二级碎片信息、参考文献以及对照品信息进行结合,找出口服广陈皮药液后吸收入血的原型成分和代谢产物。结果显示2、4、8年广陈皮鉴定出成分分别为34、24和15个,2年广陈皮黄酮类入血成分含量高于4、8年广陈皮。UHPLC-Q-Orbitrap HRMS能快速、精确、全面地分析广陈皮给药后的血中移行成分,为后期广陈皮的药效物质基础规律研究提供参考,为陈久者良科学内涵的研究提供新思路。

To analyze and identify the blood components of Citri Reticulatae Pericarpium of different aging time by ultra-performance liquid chromatography-quadrupole-electrostatic field orbitrap high resolution mass spectrometry(UHPLC-Q-Orbitrap HRMS).UHPLC-Q-Orbitrap HRMS technology and Compound Discover 3.0 software were used for analysis.The spectrum differences of Citri Reticulatae Pericarpium extract,administered serum,and blank serum were compared,and molecular weight,secondary fragment information,reference literature,and reference substance information were combined to find out the prototype components and metabolites absorbed into the blood after oral administration of Citri Reticulatae Pericarpium liquid.34,24 and 15 components were identified in the blood of 2,4 and 8-year-old Citri Reticulatae Pericarpium,and the content of flavonoids in the blood of 2-year-old Citri Reticulatae Pericarpium was higher than that of 4 and 8-year-old Citri Reticulatae Pericarpium.The UHPLC-Q-Orbitrap HRMS can provide a rapid,accurate and comprehensive analysis of the migrating components in blood after the administration of Citri Reticulatae Pericarpium,which can provide a reference for the later research on the pattern of the substance basis of the medicinal effects of Citri Reticulatae Pericarpium and provide new ideas for the study of the scientific connotation of the older the better of Citri Reticulatae Pericarpium.