益气解毒化瘀方治疗溃疡性结肠炎患者的疗效及其对炎症反应、免疫功能的影响

Effect of Yiqijiedu Huayu compound on patients with ulcerative colitis and its effect on inflammatory reaction and immune function

[目的]观察益气解毒化瘀方治疗溃疡性结肠炎(UC)患者的效果,并分析其对患者机体内炎症反应、免疫功能的影响。[方法]94例UC患者按照数字表法随机分为观察组与对照组,各47例。2组患者入院后均予以对症支持治疗,对照组在此基础上予以美沙拉嗪口服治疗,观察组在对照组基础上予以益气解毒化瘀方治疗。比较2组患者治疗前后中医症状积分变化,采用Mayo指数和改良Baron评分分别评估2组患者治疗前后疾病活动度及肠镜检查结果,采用酶联免疫吸附法检测2组患者治疗前后IL-6、IL-8、TNF-α等炎症反应指标水平,采用流式细胞仪检测2组患者治疗前后Th17和Treg细胞水平,同时计算Th17/Treg值。[结果]治疗前观察组与对照组患者中医症状积分、Mayo指数、改良Baron评分及炎症反应指标比较均差异无统计学意义(P>0.05),治疗后2组患者中医症状积分、Mayo指数、改良Baron评分及炎症反应指标均明显下降(P<0.05),且观察组较对照组下降更为明显(P<0.05);治疗前2组患者Th17、Treg细胞水平及Th17/Treg比较差异无统计学意义(P>0.05),治疗后2组患者Th17、Th17/Treg均明显下降(P<0.05),而Treg细胞水平明显升高,且观察组较对照组下降和升高更为明显(P<0.05)。[结论]益气解毒化瘀方不但可减轻UC患者临床症状、疾病严重和肠道黏膜损伤程度,而且还可抑制肠道炎症反应和调节免疫功能。

[Objective]To observe the clinic effect of Yiqijiedu Huayu compound on patients with ulcerative colitis(UC),and analyze its effect on the inflammatory reaction and immune function of the patients.[Methods]Ninety-four patients with UC were randomly divided into two groups according to the number table method, with 47 patients in each group.After admission, both groups accepted symptomatic and supportive treatment.The control group was treated with Mesalazine by oral administration, and the observation group was treated with Yiqijiedu Huayu compound on basis of the control group.The traditional Chinese medicine(TCM) symptom score changes of the two groups before and after treatment were compared.The disease activity and colonoscopy results of the two groups before and after treatment were respectively detected by the Mayo index and modified Baron score.The inflammation indexes such as IL-6,IL-8,TNF-α of the two groups before and after treatment were detected by enzyme-linked immunosorbent method.The levels of Th17 and Treg cells of the two groups before and after treatment were detected by flow cytometry, at the same time the values of Th17/Treg was calculated.[Results]There were no statistically significant differences in TCM symptom scores, Mayo index, modified Baron score and inflammatory response indexes between the two groups before treatment(P>0.05).After treatment, TCM symptom scores, Mayo index, modified Baron score and inflammatory response indexes of the two groups were significantly decreased(P<0.05),and the decrease in the observation group was more significant than those in the control group(P<0.05).Before treatment, there was no statistically significant difference in Th17 and Treg cell levels or Th17/Treg between the two groups(P>0.05).After treatment, Th17 and Th17/Treg levels of the two groups were significantly decreased(P<0.05),while Treg cell levels were significantly increased, and the changes in the observation group were more significant than those in the control group(P<0.05).[Conclusion]Yiqijiedu Huayu compound can not only improve the clinical symptoms, disease the severity and damage degree of intestinal mucosa in patients with UC,but also inhibit intestinal inflammation and regulate immune function.