基于生物信息学对食管鳞癌潜在枢纽基因的筛选

Screening of Hub Genes Related to the Development and Prognosis of Esophageal Squamous Cell Carcinoma Cancer Using Bioinformatics

目的:通过生物信息学分析,寻找食管鳞癌发生和转移相关的关键枢纽(hub)基因,挖掘食管鳞癌(ESCC)潜在的基因靶点。方法:从基因表达汇编(GEO)数据库中下载3个微阵列数据集(GSE161533、GSE100942、GSE45670),并进行深入分析。生物信息学方法包括差异表达基因和枢纽基因鉴定,GO及KEGG进行生物功能富集分析,蛋白-蛋白相互作用(PPI)网络构建与分析等。利用GEPIA、UCSC及Kaplan-Meier验证枢纽基因在ESCC患者中的表达情况及预后价值。结果:共筛选出223个差异表达基因(DEGs)(包括91个上调基因,132个下调基因)和10个hub基因(ASPM、DTL、CDKN3、AURKA、UBE2C、TPX2、TRIP13、TOP2A、CEP55和CDC6);hub基因在ESCC组织中均有高表达;其中ASPM、DTL、CDKN3、AURKA、TPX2、TOP2A和CDC6基因水平与ESCC预后相关。结论:筛选出的hub基因将为识别ESCC的诊断和预后关键标志物提供依据。

Objective:To find the key hub genes associated with the occurrence and metastasis of ESCC and to explore the potential gene targets of ESCC through bioinformatics analysis.Methods:We downloaded 3 microarray data sets(GSE161533,GSE100942,GSE45670)from the GEO database and conducted an in-depth analysis.Bioinformatics methods included identification of differentially expressed genes and hub genes,biological function enrichment analysis by GO and KEGG,and PPI network construction and analysis.GEPIA,UCSC,and Kaplan-Meier were used to verify the expression and prognostic value of hub genes in ESCC patients.Results:A total of 223 DEGs(including 91 up-regulated genes and 132 down-regulated genes)and 10 hub genes(ASPM,DTL,CDKN3,AURKA,UBE2C,TPX2,TRIP13,TOP2A,CEP55,and CDC6)were screened.The hub genes are highly expressed in ESCC tissues.Among them,the expression levels of ASPM,DTL,CDKN3,AURKA,TPX2,TOP2A,and CDC6 were correlated with the prognosis of ESCC.Conclusion:The hub genes selected in this study will provide a basis for further research on the key markers for the diagnosis and prognosis of ESCC.