松针在暗褐网柄牛肝菌母种配方优化中的应用

Application of Pine Needles in Formula Optimizing of Stock Culture of Phlebopus portentosus

为优化暗褐网柄牛肝菌母种配方,合理利用松针资源,以暗褐网柄牛肝菌为试验材料,松针浸出物为主要添加物之一,采用单因素试验和正交试验结合的方法,筛选出暗褐网柄牛肝菌的最佳母种培养基。结果表明,最佳培养基配方为松针浸出液25 g·L^(-1)、葡萄糖10 g·L^(-1)、马铃薯100 g·L^(-1)、蛋白胨2 g·L^(-1)、琼脂粉16 g·L^(-1)。培养的暗褐网柄牛肝菌菌丝黄色、生长势强,菌丝粗壮,生长速率达到3.41 mm·d^(-1)。

In order to optimize the formula of the stock culture of Phlebopus portentosus and make rational use of pine needle resources,taking P.portentosus as the experimental material and pine needle extract as one of the main additives,the best stock culture medium of P.portentosus was selected by the combination of single factor test and orthogonal test.The results showed that,the best medium were 25 g·L^(-1) of pine needle extract,10 g·L^(-1) of glucose,100 g·L^(-1) of potato,2 g·L^(-1) of peptone and 16 g·L^(-1) of agar powder.The cultured mycelium of P.portentosus was yellow,growth potential,strong mycelium,and the growth rate reached 3.41 mm·d^(-1).