2型糖尿病患者miR⁃375、miR⁃126表达水平及与胰岛素抵抗关系

Expression levels of miR⁃375 and miR⁃126 in patients with type 2 diabetes mellitus and their relationship with insulin resistance

目的探究2型糖尿病(T2DM)患者微小RNA⁃375(miR⁃375)、miR⁃126表达水平及其与胰岛素抵抗的关系。方法选择2019年1月至2019年12月新乡医学院第一附属医院收治的80例T2DM患者,根据空腹血糖(FPG)及口服葡萄糖耐量试验(OGTT)结果分为T2DM前期组32例、T2DM组48例,另选择血糖、血脂正常的40例健康人为对照组,检测各组miR⁃375、miR⁃126、空腹血糖(FPG)、空腹血胰岛素(FIns)水平,计算胰岛素抵抗指数(HOMA⁃IR),分析miR⁃375、miR⁃126水平与HOMA⁃IR的关系。结果FPG、餐后2h血糖(2hPG)、HOMA⁃IR、甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL⁃C)、FIns及miR⁃375表达水平:对照组<T2DM前期组<T2DM组,差异有统计学意义(P<0.05)。高密度脂蛋白胆固醇(HDL⁃C)水平:对照组>T2DM前期组>T2DM组,差异有统计学意义(P<0.05)。T2DM前期组、T2DM组miR126表达水平低于对照组,差异有统计学意义(P<0.05),但T2DM前期组、T2DM组miR126表达水平比较差异无统计学意义(P>0.05)。T2DM患者miR⁃375表达与FPG、2hPG、FIns、HOMA⁃IR呈正相关(r=0.461,0.523,0.506,0.624,P<0.05),miR126表达与FPG、2hPG、FIns、HOMA⁃IR呈负相关(r=-0.349,-0.457,-0.423,-0.439,P<0.05)。miR⁃375表达上调、miR126表达下调是影响T2DM患者HOMA⁃IR的危险因素(P<0.05)。结论T2DM患者血浆miR⁃375表达上调、miR⁃126表达下调,且表达水平与胰岛素抵抗密切相关。

Objective To investigate the expression levels of microRNA⁃375(miR⁃375)and miR⁃126 in patients with type 2 diabetes mellitus(T2DM)and their relationship with insulin resistance.Methods A total of 80 patients with T2DM admitted to the First Affiliated Hospital of Xinxiang Medical University were selected from January 2019 to December 2019,including the pre⁃T2DM group(32 cases)and the T2DM group(48 cases).Another 40 healthy people with normal blood glucose and blood lipids were selected as the control group.The levels of miR⁃375,miR⁃126,fasting plasma glucose(FPG)and fasting blood insulin(FIns)were detected in each group.The homeostasis model assessment of insulin resistance(HOMA⁃IR)was calculated,and the relationship between levels of miR⁃375 and miR⁃126 and HOMA⁃IR was analyzed.Results The levels of FPG,2h postprandial blood glucose(2hPG),HOMA⁃IR,triglyceride(TG),total cholesterol(TC),low⁃density lipoprotein cholesterol(LDL⁃C)and FIns and expression of miR⁃375 were shown as control group<pre⁃T2DM group<T2DM group,and the level of high⁃density lipoprotein cholesterol(HDL⁃C)was shown as control group>pre⁃T2DM group>T2DM group(P<0.05),and the expression of miR126 in the pre⁃T2DM group and T2DM group was lower than that in the control group(P<0.05).The expression of miR⁃375 in patients with T2DM was positively correlated with FPG,2hPG,FIns and HOMA⁃IR(r=0.461,0.523,0.506,0.624,P<0.05),and expression of miR126 was negatively correlated with FPG,2hPG,FIns and HOMA⁃IR(r=-0.349,-0.457,-0.423,-0.439,P<0.05).The up⁃regulated expression of miR⁃375 and the down⁃regulated expression of miR126 were risk factors for HOMA⁃IR in T2DM patients(P<0.05).Conclusion Plasma miR⁃375 expression is up⁃regulated and miR⁃126 expression is down⁃regulated in patients with T2DM,and the expression level is closely related to insulin resistance.