慢病毒介导的HNRNPK下调对肝癌细胞HepG2增殖及迁移的影响

Effect of HNRNPK downregulation mediated by lentivirus infection on HepG2 cell proliferation and migration

目的构建慢病毒介导HNRNPK下调的HepG2细胞稳转株,研究HNRNPK沉默对肝癌细胞增殖与迁移功能的影响。方法以构建的慢病毒感染HepG2细胞并筛选阳性细胞获得稳定表达的细胞株,并检测HNRNPK表达效率。通过CCK-8法与细胞克隆形成实验研究HNRNPK下调对细胞增殖的影响。通过划痕实验与Transwell实验研究HNRNPK下调对细胞迁移的影响。结果成功构建了慢病毒介导HNRNPK沉默的HepG2细胞稳转株,HNRNPK沉默显著抑制了HepG2细胞的增殖与迁移能力。结论HNRNPK下调能够在体外抑制HepG2细胞的增殖与迁移。

Objective To establish a HepG2 cell line with downregulated heterogeneous nuclear ribonucleoprotein K(HNRNPK)mediated by lentivirus and study the effects of HNRNPK downregulation on cell proliferation and migration.Methods HepG2 cells were infected with packaged lentivirus,and positive cells were selected to obtain stable transgenic cell lines.Then detected expression efficiency of HNRNPK.The effect of HNRNPK downregulation on cell proliferation was detected using cell counting CCK-8 and colony formation assays.The effect of HNRNPK downregulation on cell migration was detected using the wound healing and Transwell assays.Results The HepG2 cell line with downregulated HNRNPK mediated by lentivirus infection was successfully constructed.HNRNPK-downregulation inhibited the proliferation and migration of HepG2 liver cells.Conclusions HNRNPK-downregulation inhibits the proliferation and migration of HepG2 liver cells in vitro.