大豆Glyma.18G261700基因生物学分析及功能初探

Cloning and Biological Function Analysis of Soybean Glyma.18G261700 Gene

MYB是参与花青素合成的重要调控基因,数量众多且功能多样。为分析大豆中与花生黑色种皮调控基因AhTc1(MYB家族)同源基因的功能,本研究根据AhTc1基因序列信息,同源克隆大豆MYB家族基因Glyma.18G261700,对其进行生物信息学分析,并检测不同种皮颜色大豆苗期不同部位花青素和基因表达量。结果显示:Glyma.18G261700全长1572 bp,编码189个氨基酸,属于R2R3型MYB。AhTc1编码的蛋白为疏水性蛋白,而Glyma.18G261700与之不同,编码的蛋白为亲水性蛋白。三级结构中Glyma.18G261700与AhTc1尾部有所区别,在调控种皮颜色中可能具有不同功能。不同种皮颜色大豆幼苗根部花青素含量较低,而Glyma.18G261700基因的表达量较高,表明在大豆幼苗期该基因的大量表达可能抑制根部花青素的合成,但对种皮颜色的调控还需进一步探索。

MYB is an important regulatory gene involved in the synthesis of anthocyanins,with numerous and diverse functions.In order to analyze the function of the homologous genes of AhTc1(MYB family)in soybean and the regulatory gene of black seed coat of peanut,this study cloned the MYB family gene Glyma.18 G261700 based on the sequence information of AhTc1 gene,carried out the analysis of biological information on Glyma.18 G261700,and detected anthocyanin and gene expression in different parts of soybean seedlings with different seed coat colors.The results showed that Glyma.18 G261700 gene was 1572 bp full length,encoded 189 amino acids,and was belonged to R2 R3-MYB.The protein encoded by AhTc1 was a hydrophobic protein,but the protein encoded by Glyma.18 G261700 was a hydrophilic protein.In the tertiary structure,the tails of Glyma.18 G261700 and AhTc1 were different,and may have different functions in regulating the color of the seed coat.The root anthocyanin content in roots of soybean seedlings with different seed coat colors was lower,while the expression of the Glyma.18 G261700 gene in their roots tended to be higher.The results showed that the high expression of this gene inhibited anthocyanin synthesis in soybean roots at seedling stage,but the regulation of seed coat color should be further explored.