长链非编码RNA Linc01555在结直肠癌中的表达及生物学功能研究

Expression of long non-coding RNA Linc01555 in colorectal cancer and its biological function study

目的:探讨长链非编码RNA Linc01555在结直肠癌中的表达并分析其与患者临床病理资料及生物学功能的相关性。方法:选取100例结直肠癌组织及癌旁正常组织,采用TCGA数据库分析Linc01555在癌组织及癌旁组织的表达情况,并分析Linc01555水平与临床病理资料的相关性。结直肠癌细胞进行siRNA转染处理,将过表达lncRNA Linc01555质粒和空载质粒转入细胞中,分别记为sh-NC组、sh-Linc01555组,采用CCK-8法检测Linc01555对癌细胞增殖的影响,流式细胞术测定细胞凋亡率,Transwell实验检测癌细胞迁移和侵袭情况。结果:Linc01555在结直肠癌组织中的表达显著高于癌旁组织,Linc01555的表达水平与临床分期、病理分级、淋巴结转移等临床病理资料之间存在显著的统计学差异(P<0.05);Linc01555干扰后,结直肠癌细胞的增殖活力增加,高于sh-NC组(P<0.05),结直肠癌细胞的凋亡率降低,低于sh-NC组(P<0.05),结直肠癌细胞的迁移细胞数及侵袭细胞数增加,高于sh-NC组(P<0.05)。结论:Linc01555在结直肠癌患者癌组织中表达水平较高,并且其水平与临床分期、病理分级、淋巴结转移等临床病理资料之间存在显著相关性,还能够抑制结直肠癌细胞增殖、迁移和侵袭,促进结直肠癌细胞凋亡。Linc01555对结直肠癌的靶向治疗及预后具有重要意义,可作为新型治疗靶点。

Objective:To explore the expression of long non coding RNA linc01555 in colorectal cancer(CRC)and to analyze its correlation with clinicopathological data of patients and biological functions.Methods:Linc01555 levels in cancer tissues and adjacent tissues were analyzed by TCGA database.The association between linc01555 levels and clinicopathological data was analyzed.Colorectal cancer cells were transfected with siRNA,and the overexpressed lncRNA Linc01555 plasmid and the no-load plasmid were transferred into the cells,which were labeled as sh-NC group and sh-LINC01555 group,respectively.The effect of linc01555 on cancer cell proliferation was assessed by CCK-8 assay.The apoptosis rate was measured by flow cytometry.Cancer cell migration and invasion were detected by Transwell assay.Results:Linc01555 expression was significantly higher in colorectal cancer tissues than in adjacent non cancerous tissues.Significant statistical differences(P<0.05)were observed between linc01555 expression levels and clinicopathological data such as clinical stage,pathological grade and lymph node metastasis.After Linc01555 interference,the proliferation activity of colorectal cancer cells increased,which was higher than that of sh-NC group(P<0.05),and the apoptosis rate of colorectal cancer cells decreased,which was lower than that of sh-NC group(P<0.05).The number of migrating cells and invasive cells increased,which was higher than that of sh-NC group(P<0.05).Conclusion:Linc01555 has a high expression level in colorectal cancer tissues,and its level is significantly correlated with clinical stage,pathological grade,lymph node metastasis and other clinicopathological data.Linc01555 can also inhibit the proliferation,migration and invasion of colorectal cancer cells and promote apoptosis of colorectal cancer cells.These results indicate that Linc01555 is of great significance in the targeted therapy and prognosis of colorectal cancer,and can be used as a new therapeutic target.