利用非靶向和靶向代谢组学鉴别荆条蜜、枣花蜜和洋槐蜜

Untargeted and targeted metabolomics to discrimination of chaste,jujube and acacia honey

蜂蜜品种的鉴别是确定蜂蜜独有特性、促进优质优价的有效手段,在前期已经建立特色蜂蜜鉴别和功能识别可靠模型的基础上,本文建立了同时鉴别荆条蜜、枣花蜜和洋槐蜜的方法。采用超高效液相色谱-飞行时间质谱(UHPLC/Q-Tof-MS)技术对荆条蜜、枣花蜜和洋槐蜜3种大宗蜂蜜进行了非靶向代谢组学分析,结合正交偏最小二乘判别分析(orthogonal partial least-squares discrimination analysis,OPLS-DA)对该3种蜂蜜进行差异比较,发现咖啡酸、4-羟基喹啉和刺槐苷对3种蜂蜜的鉴别起到关键作用。采用超高效液相色谱—三重四极杆质谱(UHPLC-QqQ-MS/MS)技术建立了同时测定蜂蜜中咖啡酸、4-羟基喹啉和刺槐苷的方法,该方法简便快速、准确可靠。分析北京及周边地区荆条蜜、枣花蜜和洋槐蜜样品中的咖啡酸、4-羟基喹啉和刺槐苷的含量,确定咖啡酸、4-羟基喹啉和刺槐苷可分别作为荆条蜜、枣花蜜和洋槐蜜的标志性成分,其阈值分别为1.6mg/kg、1.0mg/kg和0.4mg/kg。

The identifi cation of honey varieties is an effective way to determine the unique characteristics of honey and promote its high quality and high price.On the base of the reliable model of characteristic honey and its function identifi cation established in the previous study,this paper developed a method to identify chaste,jujube and acacia honey.In this study,chaste,jujube and acacia honey samples were analyzed by UHPLC/Q-Tof-MS.Orthogonal partial least-squares discrimination analysis(OPLS-DA)was utilized to discriminate honey samples from different plant origins.The results showed that caffeic acid,4-hydroxyquinoline and robinin played a key role in the identifi cation of the 3 kinds of honey.A quantitative method for simultaneous determination of caffeic acid,4-hydroxyquinoline and robinin in honey was established by UHPLC-QQQ-MS/MS.The method was simple,fast,accurate and reliable.The method was simple,fast and accurate.It was used to determine the contents of caffeic acid,4-hydroxyquinoline and robinin in honey samples including chaste,jujube and acacia honey collected from Beijing and other region of China.The results showed that caffeic acid,4-hydroxyquinoline and robinin could be used as marker of chaste honey,jujube honey and acacia honey,respectively.The threshold of caffeic acid,4-hydroxyquinoline and robinin was 1.6 mg/kg,1.0 mg/kg and 0.4 mg/kg,respectively.