百会穴留针对丙戊酸钠致孤独症模型大鼠焦虑样行为作用观察

Effect of Acupuncture at Baihui(DU20)on Anxiety-Like Behavior of Autistic Rats Induced by Valproic Acid

目的观察百会穴留针对孤独症模型大鼠焦虑样行为学的影响。方法采用丙戊酸钠(valproic acid,VPA)600 mg/kg注射SD大鼠孕鼠,造仔鼠孤独症样模型。实验组别主要为空白对照组、VPA组、百会针刺治疗组。经9 d连续治疗后,在仔鼠出生后80 d开始应用旷场实验、埋珠实验、三箱社交、新物体识别实验、大鼠自我梳理统计等观察3组大鼠的行为学变化。结果①VPA模型组与空白对照组比较:模型组在旷场实验中探究社交行为明显减少(P<0.005),埋珠实验中埋珠率降低(P<0.005),三箱社交实验中社交能力明显降低(P<0.05),新物体识别中对有物体区的探索明显下降(P<0.05),自我梳理毛发次数检测中梳理次数显著增加(P<0.001)。多指标显示仔鼠孤独症模型造模成功,焦虑样行为明显。②百会针刺组与VPA组比较:百会针刺组在埋珠实验中埋珠率明显增加(P<0.05),三箱社交实验中社交能力稍有增强,但差异无统计学意义(P>0.05),新物体识别中对有物体区探索明显增加(P<0.05),自我梳理毛发次数检测中梳理次数明显减少(P<0.05)。多指标显示百会穴留针可显著改善孤独症模型仔鼠的孤独症相关焦虑样行为。结论VPA造模大鼠刻板行为增加、社交能力下降,尤其焦虑样症状明显,符合孤独症标准;在脑发育关键期内针刺百会穴可明显改善VPA模型大鼠刻板、焦虑样行为,而对其社交能力则改善不明显。

Objective To observe the effect of acupuncture at Baihui(DU20)on anxiety-like behavior of autistic rats.Methods In this experiment valproic acid(VPA,600 mg/kg)was injected into pregnant SD rats to establish the autism like model.The experimental groups were blank control group,VPA group and Baihui acupuncture treatment group.After 9 days of continuous treatment,the behavioral changes of three groups of rats were observed in two and a half months after birth by open field experiment,buried bead experiment,three boxes of social interaction,new object recognition experiment and rats’self grooming statistics.Results①Compared with the control group,VPA group showed significantly decrease in exploratory behavior(P<0.005)in the open field experiment,a decrease in the rate of embedding beads(P<0.005),a weak social ability(P<0.05)in the three box social experiment,a significant decrease in the exploration of objects areas in the recognition of new objects(P<0.05),and a significant increase in the number of self-grooming numbers(P<0.001).The results showed that the model of autism in offspring rats was successful.②Compared with VPA group,Baihui acupuncture group increased the behavior of inquiry in the open field experiment,the rate of embedding beads in the open field experiment(P<0.05),there was no significant difference in social ability in the three boxes social experiment(P>0.05),and the exploration of the object area in the new object recognition increased significantly(P<0.05).The number of self-grooming decreased significantly(P<0.05).Multiple indicators showed that Baihui(DU20)could significantly improve the autism related behaviors of the offspring of the autism model.Conclusion In VPA model rats,stereotyped behavior increased and social ability decreased,especially anxiety like symptoms,which were in line with the autism standard;in the critical period of brain development,acupuncture at Baihui point(DU20)could significantly improve the stereotyped and anxiety like behavior of VPA model rats,but the improvement of social ability was not obvious.