绒毛白蜡松散型胚性愈伤组织的诱导

Study on the Induction of Loose Embryogenic Callus from Fraxinus velutina Torr

以绒毛白蜡组培苗茎段为外植体,建立绒毛白蜡松散型胚性愈伤组织诱导体系,为绒毛白蜡遗传转化与离体诱变等研究奠定基础。试验结果显示:幼嫩茎段在WPM+0.5 mg·L^(-1)2,4-D+5 mg·L^(-1)GA_(3)+30 g·L^(-1)蔗糖的培养基上可诱导出理想的松散型愈伤组织;将松散型愈伤组织在MS+0.5 mg·L^(-1)2,4-D+1.0 mg·L^(-1)KT+30 g·L^(-1)蔗糖的培养基上继代培养,每15 d继代1次,继代时选择结构松散质地较硬的愈伤组织,3次继代培养后,可获得绒毛白蜡松散型胚性愈伤组织。

Using the stem segments of tissue cultured seedlings of Fraxinus velutina Torr,as explants,the loose embryogenic callus induction system of Fraxinus velutina was established,which laid a foundation for the study of genetic transformation and in vitro mutagenesis of Fraxinus velutina.The results showed that the young stem segments could induce ideal Loose callus on the medium of WPM+0.5 mg·L^(-1)2,4-D+5 mg·L^(-1)GA_(3)+30 g·L^(-1) sucrose;The loose callus was subcultured on the medium of MS+0.5 mg·L^(-1)2,4-D+1.0 mg·L^(-1)KT+30 g·L^(-1) sucrose,and subcultured once every 15 days.The callus with loose structure and hard texture was selected during subculture.After subculture for 3 times,the loose embryogenic callus of Fraxinus velutina Torr could be obtained.