川芎嗪改善脊髓完全横断大鼠血液流变学指标的动态观察

Tetramethylpyrazine improves hemorheological indexes in rats with complete spinal cord transection:a dynamic observation

背景:脊髓损伤后血液流变学指标的改变会引起微循环障碍,诱导组织缺血缺氧,加剧脊髓组织继发性损伤。目的:观察川芎嗪干预脊髓完全横断大鼠后不同时间节点血液流变学指标的变化。方法:将54只8周龄雌性SD大鼠随机分为假手术组、模型组、川芎嗪组,每组18只。假手术组仅行椎板切除术,模型组、川芎嗪组采用自制双刃显微剪行T_(10)脊髓完全横断,缺损间隙2 mm,川芎嗪组在模型制备后给予200 mg/(kg·d)盐酸川芎嗪注射液腹腔注射,连续5 d。分别于造模术后7,14,28 d进行腹主动脉取血,检测全血黏度(低、中、高切边率)、血浆黏度、红细胞压积、红细胞聚集指数、红细胞变形指数、红细胞刚性指数、红细胞电泳指数。结果与结论:①脊髓损伤后第7,14,28天,模型组全血黏度(低、高、中切变率)和血浆黏度均显著高于假手术组(P<0.05);第14天,模型组红细胞压积显著高于假手术组(P<0.05),红细胞刚性指数、红细胞电泳指数显著低于假手术组(P<0.05);②脊髓损伤后第7天,川芎嗪能够显著降低全血黏度(低、高、中切变率)和血浆黏度(P<0.05);第14天川芎嗪能够升高红细胞变形指数(P<0.05),一定程度上也能改善血液黏度相关指标,但差异无显著性意义(P>0.05);第28天川芎嗪能够显著改善全血黏度(低切)和血浆黏度(P<0.05);③脊髓损伤后,全血黏度及血浆黏度均会升高,红细胞相关指数也会出现不同程度的变化,川芎嗪的使用能够改善早期血液流变学指标的恶化,对于改善微循环障碍具有一定的促进作用。

BACKGROUND:Hemorheological changes following spinal cord injury can cause microcirculation disorder,induce tissue ischemia and hypoxia,and aggravate the secondary injury to spinal cord tissue.OBJECTIVE:To observe the changes in hemorheological indexes at different time points after tetramethylpyrazine intervention in rats with complete spinal cord transection.METHODS:Fifty-four 8-week-old female Sprague-Dawley rats were randomly divided into three groups(n=18 per group):sham operation group,model group,and tetramethylpyrazine group.Only laminectomy was performed in the sham operation group,while the T_(10)segment was completely transected with a 2 mm defect gap in the model and tetramethylpyrazine groups by a self-made double-edge microshear.After model preparation,tetramethylpyrazine group was given intraperitoneal injection of tetramethylpyrazine hydrochloride,200 mg/kg per day,for 5 consecutive days.Abdominal aorta blood samples were taken 7,14,and 28 days after operation for measuring whole blood viscosity(low,high and medium shear rates),plasma viscosity,hematocrit,erythrocyte aggregation index,erythrocyte deformability index,erythrocyte rigidity index,and erythrocyte electrophoresis index.RESULTS AND CONCLUSION:The whole blood viscosity(low,high,and medium shear rates)and plasma viscosity in the model group were significantly higher than those in the sham group at 7,14,and 28 days after spinal cord injury(P<0.05).Hematocrit was significantly higher but erythrocyte rigidity index and erythrocyte electrophoresis index were significantly lower in the model group than the sham group at 14 days after spinal cord injury(P<0.05).Tetramethylpyrazine significantly reduced whole blood viscosity(low,high,and medium shear rates)and plasma viscosity at 7 days after spinal cord injury(P<0.05).Tetramethylpyrazine significantly increased erythrocyte deformability index(P<0.05)and certainly but not significantly improved blood viscosity at 14 days after spinal cord injury(P>0.05).Tetramethylpyrazine significantly improved whole blood viscosity(hyposhear)and plasma viscosity at 28 days after spinal cord injury(P<0.05).To conclude,after spinal cord injury,the whole blood viscosity and plasma viscosity can increase and erythrocyte indexes can also change to varying degrees.The use of tetramethylpyrazine can improve the deterioration of early hemorheological indexes and improve microcirculation disorders.