不同卵巢储备女性性激素受体表达及其相关性

Expressions and correlation of anti-Mullerian hormone type Ⅱ receptor,granulosa cell follicle stimulating hormone receptor,and luteinizing hormone receptor of women with different ovarian reserve

目的:探讨不同卵巢储备女性卵泡液及颗粒细胞中抗苗勒管激素Ⅱ型受体(AMHRⅡ)、促卵泡刺激素受体(FSHR)、促黄体生成素受体(LHR)表达及其相关性。方法:选取2016年12月30日-2019年5月30日在海南省妇女儿童医学中心行体外受精-胚胎移植女性110例,根据患者卵巢功能分为卵巢功能正常组(43例)、卵巢功能减退(DOS)组(37例)、卵巢早衰(POF)组(30例)。获取各组卵泡液及颗粒细胞,采用qRT-PCR法检测AMHRⅡ、FSHR、LHR m RNA的相对表达量。结果:POF组年龄、体质指数、卵泡刺激素(FSH)、黄体生成素(LH)高于功能正常组和DOS组,窦卵泡个数、获卵个数、AMH、雌二醇(E;)、孕酮(P)低于卵巢功能正常组和DOS组(P<0.05)。AMHRⅡ、FSHR、LHR相对表达量POF组最低,DOS组次之,功能正常组最高(P<0.05)。相关性分析显示,在功能正常组,FSHR与LHR、AMHRⅡ表达量呈正相关,LHR与AMHRⅡ表达量呈正相关(均P<0.05);在DOS组,FSHR与LHR、AMHRⅡ表达量呈正相关(P<0.05);在POF组,FSHR与AMHRⅡ表达量呈正相关(P<0.05)。结论:不同卵巢储备女性AMHRⅡ、FSHR、LHR表达量存在差异,提示其可能与卵巢储备异常的发生机制有关。

Objective: To investigate the expressions and correlation of anti-Mullerian hormone type II receptor(AMHRII), granulosa cell follicle stimulating hormone receptor(FSHR) and luteinizing hormone receptor(LHR) in follicular fluid and granulosa cells of women with different ovarian reserve. Methods: A total of 110 women undergone in vitro fertilization-embryo transfer were enrolled in this study from December 30, 2016 to May 30, 2019. These women were divided into group A(43 cases with normal ovarian function), group B(37 cases with ovarian dysfunction(DOS)), and group C(30 cases with ovarian premature aging(POF)) according to their ovarian function. The follicular fluid and granulosa cells of the women in the three groups were obtained, and the relative expressions of AMHRII, FSHR, and LHR mRNA in follicular fluid and granulosa cells were detected by qRT-PCR. Results: The age, the body mass index value, the FSH and LH levels of the women in group C were significantly higher than those of the women in group A and group B, but the number of ovarian follicles, the number of eggs obtained, and the levels of AMH, estradiol(E;), and progesterone(P) of the women in group C were significantly lower than those of the women in group A and group C(P<0.05). The relative expression levels of AMHR Ⅱ, FSHR, and LHR of the women in group C were the lowest, followed by those of the women in group B, and which of the women in group A were the highest(P<0.05). Correlation analysis showed that FSHR level of the women in group A was positively correlated with their LHR and AMHR Ⅱ expression levels, the LHR level of the women in group A was positively correlated with their AMHR Ⅱ expression level(all P<0.05). In group B, the FSHR level of the women was positively correlated with their LHR and AMHR Ⅱlevels(P<0.05).In group C,the FSHR level of the women was positively correlated with their AMHR Ⅱlevel(P<0.05).Conclusion:The expression levels of AMHRII,FSHR,and LHR of the women with different ovarian reserve are different,which may be related to the mechanism of abnormal ovarian reserve.