期刊文献+

MiR-3200-3p调控Wnt/β-catenin通路影响肝癌细胞的迁移和侵袭 被引量:3

MiR-3200-3p Affects Liver Cancer Cell Migration and Invasion by Regulating Wnt/β-Catenin Signaling Pathway
下载PDF
导出
摘要 目的探讨miR-3200-3p对肝癌细胞迁移和侵袭的影响及其可能的分子机制。方法采用RT-qPCR方法检测miR-3200-3p在正常肝细胞系HL-7702与肝癌细胞系Bel-7402中的表达;构建miR-3200-3p的慢病毒低表达载体,转染Bel-7402细胞,倒置荧光显微镜及RT-qPCR检测敲低效果;根据转染病毒质粒的不同将Bel-7402细胞分为2组:LV-control组及LV-inhibitor组。Transwell迁移和侵袭实验检测2组细胞的迁移和侵袭能力;Western blot法检测2组细胞中β-catenin、MMP2、E-cadherin蛋白的表达水平。结果与HL-7702细胞比较,Bel-7402细胞中miR-3200-3p的相对表达量显著升高(P<0.001);成功建立miR-3200-3p低表达的Bel-7402稳转细胞系;与LV-control组比较,LV-inhibitor组肝癌细胞迁移和侵袭能力均明显受到抑制(均P<0.001),β-catenin(总、胞核和胞质)和MMP2蛋白表达均显著下调,E-cadherin的表达明显上调(均P<0.05)。结论miR-3200-3p在肝癌细胞中发挥促癌作用,敲低其表达可抑制肝癌细胞的迁移和侵袭,其机制可能是通过影响Wnt/β-catenin通路的活化而下调MMP2表达,上调E-cadherin而实现的。 Objective To explore the effect of miR-3200-3p on liver cancer cell migration and invasion and its possible mechanisms.Methods The expression of miR-3200-3p in human normal liver cell line HL-7702 and human hepatocellular carcinoma cell line Bel-7402 was detected by RT-qPCR.The low-expressed miR-3200-3p lentiviral vectors(LV)were constructed and transfected into Bel-7402 cells.The transfection efficiency was verified by RT-qPCR and inverted fluorescence microscopy.The Bel-7402 cells were divided into two groups:LV-control group and LV-inhibitor group.The migration and invasion ability of cells was measured by transwell assays,and the protein expression levels ofβ-catenin,MMP2 and E-cadherin were detected by Western blotting.Results The relative expression of miR-3200-3p in Bel-7402 cells was higher than that in HL-7702 cells(P<0.001).The stably transfected Bel-7402 cell line with low miR-3200-3p expression was successfully established.Compared with LV-control group,the migration and invasion were inhibited,the total,nuclear and cytoplasmicβ-catenin expression and the total MMP2 expression were reduced,and the E-cadherin expression was increased in LV-inhibitor group(P<0.001).Conclusion miR-3200-3p plays a carcinogenic role in hepatocellular carcinoma cells.Knockdown of miR-3200-3p can inhibit the migration and invasion of hepatocellular carcinoma cells,and its mechanisms may be involved in affecting the activation of Wnt/β-catenin pathway and thereby down-regulating MMP2 expression and up-regulating E-cadherin expression.
作者 冯豆 张洪 范月莹 宋玲 谭佳杰 FENG Dou;ZHANG Hong;FAN Yue-ying;SONG Ling;TAN Jia-jie(Department of Pharmacy,People’s Hospital,Wuhan University,Wuhan 430060,China;Grade 2019,Graduate School,Wuhan University,Wuhan 430060,China)
出处 《南昌大学学报(医学版)》 2022年第2期14-19,共6页 Journal of Nanchang University:Medical Sciences
基金 湖北省自然科学基金(2015CKB757)。
关键词 miR-3200-3p 肝细胞癌 WNT/Β-CATENIN通路 迁移 侵袭 miR-3200-3p hepatocellular carcinoma Wnt/β-catenin pathway migration invasion
  • 相关文献

参考文献2

二级参考文献76

  • 1Llovet JM, Burroughs A, Bruix J. Hepatocellular carcinoma.Lancet 2003; 362: 1907-1917 [PMID: 14667750 DOI: 10.1016/S0140-6736(03)14964-1].
  • 2El-Serag HB. Hepatocellular carcinoma. N Engl J Med 2011; 365:1118-1127 [PMID: 21992124 DOI: 10.1056/NEJMra1001683].
  • 3Llovet JM, Bruix J. Molecular targeted therapies in hepatocellularcarcinoma. Hepatology 2008; 48: 1312-1327 [PMID: 18821591DOI: 10.1002/hep.22506].
  • 4Llovet JM, Ricci S, Mazzaferro V, Hilgard P, Gane E, Blanc JF,de Oliveira AC, Santoro A, Raoul JL, Forner A, Schwartz M, PortaC, Zeuzem S, Bolondi L, Greten TF, Galle PR, Seitz JF, BorbathI, Haussinger D, Giannaris T, Shan M, Moscovici M, Voliotis D,Bruix J. Sorafenib in advanced hepatocellular carcinoma. N EnglJ Med 2008; 359: 378-390 [PMID: 18650514 DOI: 10.1056/NEJMoa0708857].
  • 5Kudo M. Current status of molecularly targeted therapy forhepatocellular carcinoma: clinical practice. Int J Clin Oncol 2010;15: 242-255 [PMID: 20509038 DOI: 10.1007/s10147-010-0089-y].
  • 6Laurent-Puig P, Zucman-Rossi J. Genetics of hepatocellulartumors. Oncogene 2006; 25: 3778-3786 [PMID: 16799619 DOI:10.1038/sj.onc.1209547].
  • 7Nejak-Bowen KN, Monga SP. Beta-catenin signaling, liverregeneration and hepatocellular cancer: sorting the good from thebad. Semin Cancer Biol 2011; 21: 44-58 [PMID: 21182948 DOI:10.1016/j.semcancer.2010.12.010].
  • 8Clevers H, Nusse R. Wnt/兝-catenin signaling and disease. Cell 2012;149: 1192-1205 [PMID: 22682243 DOI: 10.1016/j.cell.2012.05.012].
  • 9Lee HC, Kim M, Wands JR. Wnt/Frizzled signaling inhepatocellular carcinoma. Front Biosci 2006; 11: 1901-1915[PMID: 16368566].
  • 10Giles RH, van Es JH, Clevers H. Caught up in a Wnt storm: Wntsignaling in cancer. Biochim Biophys Acta 2003; 1653: 1-24 [PMID:12781368].

共引文献94

同被引文献17

引证文献3

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部