木犀草素通过调控内质网应激抑制平滑肌细胞的迁移

Luteolin Inhibits HASMCs Migration by Endoplasmic Reticulum Stress

目的 研究木犀草素对人主动脉平滑肌细胞迁移的调控作用,并探讨其调控作用是否通过激活内质网应激途径实现。方法 体外培养人主动脉平滑肌细胞,分为空白对照组(不做任何处理,BC)、阴性对照组(加等量无关试剂,NC)、木犀草素组(25μmol/L处理)、木犀草素+4-苯基丁酸钠共处理组(4-苯基丁酸钠预处理后加入等量木犀草素),于12h小时后细胞划痕实验法检测人主动脉平滑肌细胞迁移情况,Western blot和RTqPCR检测BIP、CHOP蛋白及RNA表达水平。结果 (1)与空白对照组和阴性对照组相比,木犀草素组细胞迁移水平明显降低,BIP、CHOP表达水平明显增高;(2)与木犀草素组相比,木犀草素+4-苯基丁酸钠共处理组细胞迁移水平增强,同时BIP、CHOP表达水平降低。结论 木犀草素通过上调内质网应激抑制人主动脉平滑肌细胞的迁移。

Objective To investigate the regulatory effect and mechanism of luteolin on the migration of human aortic smooth muscle cells(HASMCs).Methods HASMCs were cultured in vitro and divided into the blank control group(without any treatment,BC),the negative control group(with the same amount of irrelevant reagent,NC),the luteolin group(25 μmol/L treatment,LUT) and the luteolin+4-PBA treatment group(with the same amout of luteolin added after 4-PBA pretreatment,4-PBA).Cell scratch test was used to detect the HASMCs migration after 12hs.The expression level of BIP and CHOP about endoplasmic reticulum stress were detected by RTq PCR and Western blot.Results (1) Compared to the BC and NC groups,the cell migration level in the LUT group was significantly reduced,and the expression levels of BIP and CHOP were significantly increased.(2) Compared with the LUT group,the cell migration level of the luteolin+4-PBA co-teatment group increased,while the expression levels of BIP and CHOP decreased.Conclusion Luteolin inhibits HASMCs migration by promoting the endoplasmic reticulum stress.