程序性死亡受体-1/程序性死亡配体-1在食管鳞状细胞癌组织中的表达及临床意义

Expression and clinical significance of programmed death-1/programmed death ligand-1 in esophageal squamous cell carcinoma

目的探究食管鳞状细胞癌组织中程序性死亡受体-1(PD-1)/程序性死亡配体-1(PD-L1)的表达水平及临床意义。方法选取2018年1月至2019年12月中一东北国际医院收治的90例食管鳞状细胞癌患者作为研究对象,所有患者均进行病理组织切片活检,并刮取患者癌旁正常组织行相同活检,使用免疫组织化学法检测食管鳞状细胞癌组织与癌旁正常组织的PD-1/PD-L1水平。比较食管鳞状细胞癌组织与癌旁正常组织的PD-1、PD-L1表达阳性率,分析食管鳞状细胞癌组织中PD-L1表达阳性率与肿瘤T分期的关系及PD-1表达水平与无进展生存期(FPS)的关系。结果食管鳞状细胞癌组织中临界值5.0%、临界值10.0%的PD-1、PD-L1表达阳性率均高于癌旁正常组织,差异有统计学意义(P<0.05)。PD-L1表达与肿瘤T分期有关(P<0.05)。PD-1表达阳性患者无进展生存期(PFS)短于PD-1表达阴性患者(P<0.05)。结论PD-1/PD-L1在食管鳞状细胞癌组织中均存在高表达,PD-L1表达与肿瘤T分期有关,可反映疾病严重程度,而PD-1表达可影响患者预后,减少患者无进展生存期。

Objective To explore the expression and clinical significance of programmed death-1(PD-1)/programmed death ligand-1(PD-L1)in esophageal squamous cell carcinoma.Methods A total of 90 patients with esophageal squamous cell carcinoma admitted to Zhongyi Northeast Inter-national Hospital from January 2018 to December 2019 were selected as the research subjects.All patients underwent pathological tissue biopsy,and the normal tissues adjacent to cancer were taken for the same biopsy.The levels of PD-1/PD-L1 in esophageal squamous cell carcinoma and adjacent normal tissues were detected by immunohistochemistry.The positive rates of PD-1/PD-L1 expression in esophageal squamous cell carcinoma and ad-jacent normal tissues were compared,the relationship between the positive rate of PD-L1 expression and T-stage of esophageal squamous cell carcino-ma,and the relationship between PD-1 expression and progression-free survival(PFS)were analyzed.Results The positive rates of PD-1 and PD-L1 with critical values of 5.0%and 10.0%in esophageal squamous cell carcinoma tissues were higher than those in normal adjacent tissues,and the differ-ences were statistically significant(P<0.05).There was a correlation between PD-L1 expression and T stage(P<0.05).The progression free survival(PFS)of patients with positive PD-1 expression was shorter than that of patients with negative PD-1 patients(P<0.05).Conclusion PD-1/PD-L1 are highly expressed in esophageal squamous cell carcinoma,PD-L1 expression is related to tumor T stage,which can reflect the severity of the disease,PD-1 expression can affect the prognosis and reduce progression free survival of patients.