参附注射液对阿霉素所致心肌毒性的保护作用研究

Protective effect of Shenfu Injection on adriamycin-induced myocardial toxicity

目的研究参附注射液对化疗药阿霉素引起的心肌毒性的保护作用。方法大鼠心肌细胞H9c2体外培养,给予不同的药物处理,分为阿霉素、参附、参附+阿霉素,以及生理盐水的对照组,通过检测细胞凋亡、线粒体氧化损伤、活性氧(ROS)水平以及凋亡关键蛋白的变化,研究参附注射液对心肌细胞保护的分子机制。结果参附注射液可以抑制阿霉素引起的心肌细胞凋亡、减少ROS的产生、改善线粒体氧化损伤,从而起到保护作用。结论参附注射液对阿霉素引起的心脏损伤有一定的保护作用。

Objective To study the protective effect of Shenfu Injection(SFI)on cardiotoxicity caused by adriam ycinchemotherapy.Methods The cardiomyocytes H9 c2 cells of rat were cultured in vitro and treated with different drugs.They were divided into several treatment groups:doxorubicin groupu,SFI grouup,SFI and doxorubicin combination group,and physiological saline control group.Cell apoptosis,mitochondrial oxidative damage,ROS level and activation of apoptosis caspase-3 were evaluated to study the molecular mechanism of Shenfu injection on cardiomyocyte protection.Results SFI can significantly inhibit the cardiomyocyte apoptosis caused by doxorubicin,reduce the production of ROS,and mitigate mitochondrial oxidative injury so as to play a protective role.Conclusion SFI certainly has a certain protective effect on heart injury caused by doxorubicin.