摘要
以柳罐枣为原料,采用响应面法优化超声波辅助提取多糖工艺,并对其进行脱蛋白、脱色处理,分析光谱性质。结果表明,柳罐枣多糖的最佳提取工艺条件为:超声功率270W,提取时间20min,提取温度70℃,在此条件下多糖得率为15.33%。各单因素对多糖得率影响的顺序为超声功率>提取温度>提取时间。红外光谱分析显示柳罐枣多糖具有典型的糖类特征吸收峰,多糖中含有糖醛酸,是一种β-吡喃型多糖。该结果为柳罐枣多糖的提取纯化、结构鉴定及生物活性研究提供了理论基础。
The process of polysaccharides extraction from Liuguan jujube with ultrasonic assistance was optimized by response surface methodology.The Liuguan jujube polysaccharides spectral properties were analyzed after deproteinization and decolorization.The optimal extraction conditions of the Liuguan jujube polysaccharides were:ultrasonic power 270W,extraction time 20min,extraction temperature 70℃.Under these conditions,the yield of polysaccharides was 15.33%.The order of influence factors on polysaccharides yield was:ultrasonic power>extraction temperature>extraction time.The infrared spectrum analysis showed that Liuguan jujube polysaccharides had typical characteristic absorption peak of sugar,which contained uronic acid andβ-pyranoid type polysaccharide.The results provide a theoretical basis for extraction,purification,structure analysis and biological activity study of Liuguan jujube polysaccharides.
作者
李楠
李桂芬
刘亚琴
张香飞
王芮东
LI Nan;LI Guifen;LIU Yaqin;ZHANG Xiangfei;WANG Ruidong(Department of Life Science,Yuncheng University,Yuncheng 044000;Science Experiment Center,Yuncheng University,Yuncheng 044000)
出处
《中国食品添加剂》
CAS
北大核心
2022年第5期57-63,共7页
China Food Additives
基金
山西省高等学校科技创新项目(2020L0567)
山西省重点学科建设经费资助项目(FSKSC)
运城学院院级科研项目(CY-2020023)。
关键词
柳罐枣
多糖
响应面法
光谱分析
提取
Liuguan jujube
polysaccharides
response surface method(RSM)
spectral analysis
extract