固定化脂肪酶TLIM催化制备低芥酸文冠果甘油酯

Preparation of Low Erucic Acid Glycerol from Xanthoceras sorbifolia Bunge by Immobilized Lipase TLIM

以固定化脂肪酶TLIM作为催化剂,以文冠果油为原料进行水解反应制备低芥酸文冠果甘油酯。通过单因素实验考察了酶添加量、反应时间、反应温度、水油摩尔比对水解反应的影响,并采用正交实验对酶水解反应条件进行了优化。结果表明:固定化脂肪酶TLIM催化制备低芥酸文冠果甘油酯的最佳反应条件为酶添加量(质量分数)5%、反应温度45℃、反应时间5 h、水油摩尔比30∶1,在该条件下文冠果甘油酯中的芥酸含量达到4.86%。证明了文冠果油的芥酸分布在甘油三酯1,3位上,神经酸含量保持在1%左右。

Lipase TLIM was used as catalyst to prepare low erucic acid glyceride from Xanthoceras sorbifolia Bunge oil.The effects of enzyme addition amount,reaction time,reaction temperature and water/oil molar ratio on hydrolysis reaction were investigated by single factor test,and the hydrolysis reaction conditions were optimized by orthogonal test.The results showed that the optimal reaction conditions for the preparation of low erucic acid triglyceride from Xanthoceras sorbifolia Bunge catalyzed by immobilized lipase TLIM were as follows:enzyme dosage of 5%,reaction temperature of 45℃,reaction time of 5 h,water/oil ratio of 30∶1.Under these conditions,the content of erucic acid in Xanthoceras sorbifolia Bunge triglyceride reached 4.86%.It was proved that erucic acid of Xanthoceras sorbifolia Bunge oil was distributed in position 1 and 3 of triglyceride,and the content of nervonic acid was kept at about 1%.