不同林龄橡胶林土壤细菌群落组成及多样性研究

Soil Bacterial Community of Rubber Plantations of Different Ages of Stand:Composition and Diversity Study

为了了解不同林龄橡胶树土壤细菌群落的组成及多样性,以5点取样法采集土壤样品,用高通量测序测得3种龄林土壤细菌群落组成、多样性及季节变化数据。酸杆菌门、绿弯菌门、放线菌门、厚壁菌门、拟杆菌门呈显著性差异,硝化螺旋菌门呈极显著性差异。酸杆菌纲、放线菌纲、γ-变形菌纲、鞘脂杆菌纲呈显著性差异,纤线杆菌纲、OPB35_soil_group、芽孢杆菌纲呈极显著性差异。norank_c__Acidobacteria、变异性杆菌属、Candidatus_koribacter、norank_o__JG30-KF-AS9呈显著性差异,norank_c__OPB35_soil_group、醋酸杆菌杆菌属呈极显著性差异。3种林龄土壤细菌群落Simpson指数、Sobs指数、Ace指数和Shannon指数无显著性差异。季节对微生物群落解释度为24.60%。变形菌和酸杆菌是最丰富的细菌群,多样性指数无显著性差异,季节是影响群落结构及多样性的重要因素。

To understand the soil bacterial composition and diversity of rubber plantations of different ages of stand, soil samples were collected with 5-point sampling method, and the composition, diversity, and seasonal changes of soil bacterial communities in forests of three ages of stand were measured by using high-throughput sequencing. Acidobacteria, Chloroflexi, Actinobacteria Firmicutes, Bacteroidetes showed significant differences, and Nitrospirae showed extremely significant differences. Acidbacteria, Actinobacteria, γ-Proteobacteria, and Sphingobactaiia showed significant differences, while Ktedonobacteria, OPB35_soil_group,and Bacilli showed extremely significant differences. norank_c__Acidobacteria, Variibacter,Candidatus_koribacter, norank_o__JG30-KF-AS9 showed significant differences, and norank_c__OPB35_soil_group and Acidibacter showed extremely significant differences. There was no significant difference in the Simpson index, Sobs index, Ace index and Shannon index among the soil bacterial communities of the forests of three ages of stand. The seasonal interpretation of the microbial community was24.60%. In summary, Proteobacteria and Acidobacteria were the most abundant bacterial groups and there was no significant difference in the diversity index, and the season was an important factor affecting community structure and diversity of soil bacterial communities of rubber plantations.