摘要
旨在明确褐飞虱取食危害胁迫下,云南元阳哈尼族水稻地方品种‘月亮谷’的化学防御生理特征。以‘月亮谷’,‘TN1’及‘RHT’稻株为材料,褐飞虱取食胁迫后,采用分光光度计法测定稻株内可溶性糖、丙二醛(MDA)、过氧化氢(H_(2)O_(2))、多酚氧化酶(PPO)和过氧化物酶(POD)的变化。褐飞虱取食胁迫3~96 h 后,‘月亮谷’稻株内可溶性糖平均含量下降 42.85%(P<0.05,下同);H_(2)O_(2)平均含量增加9.39%;MDA 平均含量增加 10.77%;多酚氧化酶(PPO)平均活性增加 49.96%;过氧化物酶(POD)平均活性下降14.75%。褐飞虱取食胁迫后,云南元阳哈尼族地方水稻品种‘月亮谷’植株内可溶性糖、丙二醛、过氧化氢和主要防御酶较感虫品种‘TN1’反应敏感,且与抗虫品种‘RHT’反应相近。本研究表明,云南哈尼族地方水稻品种‘月亮谷’可作为良好的抗褐飞虱育种材料应用于水稻育种或作为抗性品种推广种植。
The purpose of this study is to investigate the physiological characteristics of chemical defense of rice variety‘Acuce’of Hani nationality in Yuanyang of Yunnan Province under the feeding stress of brown planthopper.‘Acuce’,‘TN1’and‘RHT’were used as materials in this study, the changes of soluble sugar,malondialdehyde(MDA), hydrogen peroxide(H_(2)O_(2)), polyphenol oxidase(PPO) and peroxidase(POD) in rice plants were determined by spectrophotometer after feeding stress of brown planthopper. After 3-96 h feeding stress, the average soluble sugar content in‘Acuce’was decreased by 42.85%(P<0.05, the same below);the average content of H_(2)O_(2) was increased by 9.39%;the average content of MDA was increased by 10.77%;the average activity of polyphenol oxidase(PPO) was increased by 49.96%, while the average activity of peroxidase(POD) was decreased by 14.75%. After feeding stress of brown planthopper, the soluble sugar,malondialdehyde(MDA), hydrogen peroxide(H_(2)O_(2)) and main defense enzymes in‘Acuce’were more sensitive compared with those of the susceptible variety‘TN1’and similar to those of the resistant variety‘RHT’. This study shows that the Hani rice variety‘Acuce’in Yunnan can be used as a good breeding material for resistance to brown planthopper or be promoted as a resistant rice variety.
作者
黄钰
陈斌
肖关丽
HUANG Yu;CHEN Bin;XIAO Guanli(College of Agriculture&Biology Technology,Yunnan Agricultural University,Kunming 650201;College of Plant Protection,Yunnan Agricultural University,Kunming 650201)
出处
《中国农学通报》
2022年第9期123-129,共7页
Chinese Agricultural Science Bulletin
基金
省部共建云南生物资源保护与利用国家重点实验室开放课题“滇西南跨境空中昆虫种群结构动态研究”(gzkf201901)。
关键词
褐飞虱
水稻
月亮谷
次生产物
防御酶
防御反应
brown planthopper
rice
‘Acuce’
secondary products
defense enzyme
defense response