β-甘露聚糖酶部分水解香豆胶对自然衰老小鼠的抗衰老作用

Anti-aging Effect of Partially Hydrolyzed Fenugreek Gum byβ-Mannanase on Naturally Aging Mice

目的:研究部分水解香豆胶对自然衰老小鼠的抗衰老作用及其作用机理。方法:采用β-甘露聚糖酶水解香豆胶制备的部分水解香豆胶饮食干预自然衰老小鼠12个月,观察小鼠大脑、肝脏、肾和结肠组织病理形态学变化并测定其血清中衰老标志物及总抗氧化能力(total antioxidant capacity,T-AOC)、过氧化氢酶(catalase,CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)水平和丙二醛(malondialdehyde,MDA)水平,同时检测神经元数量、脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)及p53/p21/p16信号通路相关基因和蛋白的表达情况。结果:部分水解香豆胶能够有效提高衰老小鼠的存活率(约25.0%),明显改善其反应性、脱毛程度;T-AOC、CAT活力和GSH-Px活力较自然衰老组小鼠分别增加78.1%(P<0.05)、122.9%(P<0.05)和2.7%,而MDA浓度降低40.3%(P<0.01);增加大脑海马CA1区中神经元和BDNF阳性细胞数量,抑制肝脏p53/p21/p16信号通路中p53、p21和p16基因和p53、p16蛋白的表达。结论:部分水解香豆胶具有显著抗小鼠衰老的作用,能够抑制由衰老诱发的氧化应激和脑损伤,具有作为抗衰老功能性食品配料的潜力。

Objective:To study the anti-aging effect and underlying mechanism of partially hydrolyzed fenugreek gum(PHFG)on naturally aging mice.Methods:PHFG,prepared withβ-mannanase,was used to dietary intervention in naturally aging mice for 12 months,and then the pathological changes of brain,liver,kidney and colon tissues were observed.Furthermore,the contents of aging markers,total antioxidant capacity(T-AOC),catalase(CAT)activity,glutathione peroxidase(GSH-Px)activity and malondialdehyde(MDA)level in serum were measured.The total number of neurons,and the expression levels of brain-derived neurotrophic factor(BDNF)and p53/p21/p16 pathway associated genes and proteins were detected.Results:PHFG effectively increased the survival rate of aging mice(approximately 25.0%),and significantly improved their reactivity and depilation.The levels of T-AOC,CAT and GSH-Px in PHFG-treated mice increased by 78.1%(P<0.05),122.9%(P<0.05)and 2.7%,respectively,compared with the naturally aging ones,while the MDA content decreased by 40.3%(P<0.01).Moreover,PHFG increased the numbers of neurons and BDNF positive cells in the hippocampus CA1 area and inhibited the expression levels of the p53,p21 and p16 genes and p53 and p16 proteins in the liver.Conclusion:PHFG can prevent aging,and inhibit oxidative stress and brain damage induced by aging,which provides a scientific basis for PHFG as an anti-aging functional food ingredient.